Widespread microbial genome sequencing presents an opportunity to understand the gene regulatory networks of non-model organisms. This requires knowledge of the binding sites for transcription factors whose DNA-binding properties are unknown or difficult to infer. We adapted a protein structure-based method to predict the specificities and putative regulons of homologous transcription factors a...

An immunological approach, based on both antigen binding tests and radioimmunoassay, has been used for the comparative study of ferredoxin from Halobacterium of the Dead Sea and ferredoxins from a variety of sources. The halophilic ferredoxin was not cross-reactive with the plant, and the mammalian ferredoxins tested. On the other hand, it exhibited up to 80% of antigenic crossreactivity with a...

A method of direct lipid analysis by MALDI mass spectrometry in intact membranes, without prior extraction/separation steps, is described. The purple membrane isolated from the extremely halophilic archaeon Halobacterium salinarum was selected as model membrane. Lyophilized purple membranes were grinded with 9-aminoacridine as dry matrix and the powder mixture crushed in a mechanical die press ...

This paper views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and new aspects of their nanoand biotechnological usage. On an example of bacteriorhodopsin was described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled ...