A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Preserving the Past & Engaging Future: Theology Religion in American Special Collections is a fine collection of essays that demonstrates practical work and value special collections libraries for religion scholarship cultural heritage. Editor M. Patrick Graham giant field religious studies collections, having retired 2017 from Pitts Theological Library at Emory University after long succes...

Social Transformation in Islamic Education Perspective of HadithThe social transformation that has a bad or good impact affects various aspects, one which is the aspect education. In education, will occur harmony with progress world and development science. can have positive such as digital learning media, hybrid models, negative student moral deviation, promiscuity, hoax news, are not accordan...

The article discusses selected poetry by Darko Suvin against the background of his theoretical writings about science fiction, utopia and poetry. It argues that Suvin’s estranges ideological view present history as an inevitable reproduction injustice alienation. focus is on several poems included in collections Long March: Notes Way 1981–1984 (1987) Defined a Hollow: Essays Utopia, Science Fic...

conformity of miracle of every prophet with the prevailing art and occupation of every time is a well-known matter among scholars. such a theme is narrated for the first time through a chain of transmitters from the 10th shi‘a imam (al-hadi), though it is attributed to the 8th imam (al-rida). as the chain of transmission of the hadith is poor, investigation of its theme is necessary. in this ha...

In our review we analyze various aspects of the Russian military, shown by T. Shevchenko in stories "The Maidservant", Princess", Unhappy", Twins", Lady Captain", Artist", Walk with pleasure and not without morality" to a certan extent Musician". Namely analysis consider way life army – both current retired, their moral principles, social status, actual military component life, including educat...