High titers of interferon were induced by the psittacosis agent in guinea pig leukocyte cultures. Optimal yields of interferon were produced from freshly prepared guinea pig leukocyte cultures containing 4 x 106 cells per ml inoculated with a psittacosis/cell multiplicity of 1.0 and incubated at 35 C for 24 hr. Leukocytes obtained from 2to 4-month-old guinea pigs produced 20 times more interfer...

1. Accessory lobes of thymus, derived from the third pharyngeal pouch, occurring in close association with the parathyroids from the third pouch, were found in serial section of the cervical tissues of eleven out of fourteen guinea pigs, and probably would have been found in all fourteen but for a technical error. 2. It is probable, therefore, that accessory lobes of thymus having this situatio...

Relaxin peptide (RLN), which signals through the relaxin family peptide 1 (RXFP1) GPCR receptor, has shown therapeutic effects in an acute heart failure clinical trial. We have identified a small-molecule agonist of human RXFP1, ML290; however, it does not activate the mouse receptor. To find a suitable animal model for ML290 testing and to gain mechanistic insights into the interaction of vari...

The histological morphology of oviduct epithelia have been described well, however, the expression pattern of the gap junction proteins in the cells, and the function related with the proteins, such as [Ca2+]i dynamics pattern of living oviduct epithelia at different ages have not been clarified. We used immunohistochemistry to compare the expression pattern of gap junction proteins in the cell...

Form deprivation (FD) was induced in 61 guinea pigs with a diffuser worn on one eye. The form-deprived eye elongated and developed myopia within 6 days in animals raised under a 12:12 h light/dark cycle, but not when reared in darkness. After 11 days of FD, the average eye was -6.6 D more myopic and 146 microm longer than its fellow eye. Initially the myopia was mostly from vitreous chamber elo...

The method of competitive binding [Kaplan, Stevenson & Hartley (1971) Biochem. J. 124, 289-299] with 1-fluoro-2,4-dinitrobenzene as the labelling reagent [Duggleby & Kaplan (1975) Biochemistry 14, 5168-5175] was used to determine the chemical properties, namely pK and reactivity, of the amino groups, the histidine residues and the tyrosine residues of the dimeric form of pig zinc-free insulin a...

Expression and function of Kv7 (KCNQ) voltage-activated potassium channels in guinea pig and human airway smooth muscle cells (ASMCs) were investigated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), patch-clamp electrophysiology, and precision-cut lung slices. qRT-PCR revealed expression of multiple KCNQ genes in both guinea pig and human ASMCs. Currents with electro...

Interaction in free solution of highly purified preparations of human C'1 esterase, C'4, C'2, and C'3, in the presence of Mg(2+), resulted in rapid generation of an activity indistinguishable by biological criteria from anaphylatoxin. The formation of anaphylatoxin was associated with immunoelectrophoretic conversion of C'3 to anodically faster migrating proteins and was unaffected by the prese...

This paper considers Thomas Beddoes's role in disseminating German psychological ideas in Britain. It describes the German tradition as inaugurated by Karl Philipp Moritz (1756-93) and considers the chief differences between this tradition and the English one stemming from David Hartley. It is suggested that Beddoes found strong support for his convictions about human interiority in writings by...

BACKGROUND An estimated one fifth of all U.S. adult smokers receive health benefits through insurance plans administered by Taft-Hartley Health and Welfare Funds. Most funds do not offer comprehensive tobacco-cessation services to fund participants despite evidence that doing so would be cost effective and save lives. PURPOSE This paper examines the decision-making processes of Minnesota-base...