Asymmetric forms of acetylcholinesterase (AChE) are thought to be the predominant forms of this enzyme at vertebrate neuromuscular junctions where they attach to the synaptic basal lamina via a collagen-like tail. High salt and heparin-containing buffers are capable of solubilizing asymmetric AChE molecules from skeletal muscle; however, detachment of AChE specifically from synaptic basal lamin...

The ultrastructural characteristics of alveolar (ABM) and capillary (CBM) basement membranes in the adult rat lung have been defined using tannic acid fixation, ruthenium red staining, or incubation in guanidine HCl. ABM is dense and amorphous, has 3- to 5-nm filaments in the lamina rara externa (facing the alveolus) that run between the lamina densa and the basal cell surface of the epithelium...

The protein B of group B streptococci can bind in a nonimmune reaction to Ig of the IgG and IgM classes of various mammalian species (i.e., human, mouse, rabbit, and bovine). Protein B binding involves the Fc parts of both IgG and IgM molecules. Monoclonal or polyclonal IgG or IgM and the IgM-FC5 mu fragment of human myeloma protein combined with the protein B thereby inhibiting protein B-induc...

The physicochemical and immunological characteristics of the human tissue binding protein for 25-hydroxycholecalciferol (25-OH-D3) were studied. The serum 25-OH-D3 binding protein sedimentation in 5 to 20% linear sucrose gradients was 3 to 4 S whether detected by 25-OH-[3H]D3 binding, the radioiodination of the purified protein, or by specific radioimmunoassay. All 25-OH-[3H]D3 binding by serum...

Three patients presented a unique syndrome of recurrent panniculitis with an IgGkappa paraprotein and depletion of the early components of the classical pathway of complement. The IgGkappa paraproteins were monomers with a normal structure, and with no evidence for aggregation, as assessed by electron microscopy and ultracentrifugation. Both heavy and light chains were of normal molecular size ...

We describe a new class of potent, non-amide-based small molecule thrombin inhibitors in which an amidinohydrazone is used as a guanidine bioisostere on a non-peptide scaffold. Compound 4 exhibits nM inhibition of thrombin, is selective for thrombin, and shows 60 and 23% bioavailability in rabbits and dogs, respectively. Crystallographic analysis of 4 bound to thrombin confirmed the amindinohyd...

New wedge-shaped thermotropic liquid crystalline materials containing a guanidinium moiety at the apex organize into various supramolecular structures such as hexagonal columnar, rectangular columnar and Pm3n cubic mesophases depending on anions illustrating guest-directed self-organization in mesophases.

In the title compound, C(18)H(29)N(3)O, a polysubstituted guanidine, the torsion angles indicate that the guanidine unit and the carbonyl group are almost perpendicular to one another [O-C-N-C= -7.40 (18), C-N-C-N= -97.21 (15) and 86.41 (13)°]. The crystal packing is stablized by inter-molecular N-H⋯O hydrogen bonds, which link the mol-ecules into a chain.

BACKGROUND The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. METHODS An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested wit...

Aggregation is a serious obstacle for recovery of biologically active heterologous proteins from inclusion bodies (IBs) produced by recombinant bacteria. E. coli transformed with a vector containing the cDNA for Bothropstoxin-1 (BthTx-1) expressed the recombinant product as IBs. In order to obtain the native toxin, insoluble and aggregated protein was refolded using high hydrostatic pressure (H...