Migration-inhibitory factor (MIF) is a lymphokine that acts to localize mononuclear phagocytes (monocytes and macrophages) and perhaps to activate them. Mo cells are a human T cell leukemia virus II-infected T cell line previously shown to secrete large quantities of MIF upon stimulation with phytohemagglutinin and phorbol myristate acetate. MIF was purified from Mo cell-conditioned medium by g...

BACKGROUND Ga-labeled radiotracers are increasingly used for PET imaging. During the labeling procedure, formation of (68)Ga-colloid may occur. Upon i.v. injection, (68)Ga-colloid will accumulate rapidly in the liver, spleen, and bone marrow, resulting in reduced target-to-background ratios. In this study, we applied a thin layer chromatography (TLC) method to measure colloid content and we stu...

Objective: The aim of the present study was the development and validation of a simple, precise, rapid, accurate and specific RP-HPLC method in which the peaks will be appeared with high resolution and short period of retention time as per ICH Guidelines. Methods: Simple, sensitive, specific, accurate reversed phase high performance liquid chromatographic method was developed for the simultaneo...

Aims: To develop and validate a new, simple, rapid, precise accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of Tigecycline in bulk pharmaceutical dosage form.
 Study Design:
 Place Duration Study: RBVRR women's college pharmacy, Barkatpura, Hyderabad, between june 2019 july 2020.
 Methodology: The RP-HPLC was deve...

Objective: The present work was undertaken with the aim to develop and validate a rapid and consistent stability indicating RP-HPLC in which the peaks will be appear with short period of time as per ICH Guidelines. The proposed method was simple, fast, accurate and precise method for the Quantification of drug in the dosage form, bulk drug as well as for routine analysis in Quality control. Met...

BACKGROUND Repaglinide is a miglitinide class of antidiabetic drug used for the treatment of type 2 diabetes mellitus. A fast and reliable method for the determination of repaglinide was highly desirable to support formulation screening and quality control. OBJECTIVE UV spectrophotometric and reversed-phase high performance liquid chromatography (RP-HPLC) methods were developed for determinat...

A novel peptide having free radical scavenging activity was separated, using an on-line high-performance liquid chromatography (HPLC) - ABTS screening method, from bovine skim milk fermented by Lactococcus lactis SL6 (KCTC 11865BP). It was further purified using reverse phase-HPLC (RP-HPLC) and sequenced by RP-HPLC-tandem mass spectrometry. The amino acid sequence of the identified peptide was ...

Four peroxidase components, named RP-2, 4, 6, and 7, were isolated from rice (Oryza sativa L.) green leaves. Isoelectric focusing indicated that each preparation was homogeneous. The molecular weights of RP-2, 4, 6, and 7 estimated by SDS-PAGE were 48,000, 48,000, 40,000, and 39,500, and their isoelectric points were 5.4, 8.1, 9.3, and 9.2, respectively. The activity of every preparation was ma...

32 A toxicovenomic study was performed on the venom of the green mamba, 33 Dendroaspis angusticeps. Forty-two different proteins were identified in the venom of 34 D. angusticeps, in addition to the nucleoside adenosine. The most abundant proteins 35 belong to the three-finger toxin (3FTx) (69.2%) and the Kunitz-type proteinase inhibitor 36 (16.3%) families. Several sub-subfamilies of the 3FTxs...