نتایج جستجو برای: gp41

تعداد نتایج: 1630  

Journal: :The Journal of Experimental Medicine 1991
C F Ebenbichler N M Thielens R Vornhagen P Marschang G J Arlaud M P Dierich

Human immunodeficiency virus type 1 (HIV-1), in contrast to animal retroviruses such as murine leukemia virus, is not lysed by human complement. Nevertheless, HIV-1 activates complement via the classical pathway independent of antibody, and C3b deposition facilitates infection of complement receptor-bearing cells. Using gel exclusion chromatography on Sephacryl S-1000, purified virions were fou...

Journal: :Journal of virology 2001
C E Parker L J Deterding C Hager-Braun J M Binley N Schülke H Katinger J P Moore K B Tomer

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS), in combination with proteolytic protection assays, has been used to identify the functional epitope on human immunodeficiency virus envelope glycoprotein gp41 for the broadly neutralizing anti-gp41 human monoclonal antibody 2F5. In this protection assay-based procedure, a soluble gp140 protein with a stabilizing intermole...

Journal: :Organic & biomolecular chemistry 2012
Lei Zhao Pei Tong Yong-Xiang Chen Zhi-Wen Hu Kun Wang Yu-Ning Zhang De-Sheng Zhao Li-Feng Cai Ke-Liang Liu Yu-Fen Zhao Yan-Mei Li

HIV entry is mediated by the envelope glycoproteins gp120 and gp41. The gp41 subunit contains several functional domains: the N-terminal heptad repeat (NHR) domains fold a triple stranded coiled-coil forming a meta-stable prefusion intermediate. The C-terminal heptad repeat (CHR) subsequently folds onto the hydrophobic grooves of the NHR coiled-coil to form a stable 6-helix bundle, which juxtap...

Journal: :The Journal of Experimental Medicine 1991
Q J Sattentau J P Moore

The human immunodeficiency virus (HIV) binds to the surface of T lymphocytes and other cells of the immune system via a high affinity interaction between CD4 and the HIV outer envelope glycoprotein, gp120. By analogy with certain other enveloped viruses, receptor binding by HIV may be followed by exposure of the hydrophobic NH2 terminus of its transmembrane glycoprotein, gp41, and fusion of the...

Journal: :Biochemistry 1995
S C Blacklow M Lu P S Kim

Previous attempts to define the oligomeric state of the HIV and SIV envelope glycoproteins have yielded conflicting results. We have produced in Escherichia coli a recombinant model for the ectodomain of the SIV envelope protein gp41 and have identified a small, trimeric subdomain by proteolytic digestion of this gp41 fragment. The subdomain assembles from two peptide fragments, spanning residu...

2011
Lynn Morris Xi Chen Munir Alam Georgia Tomaras Ruijun Zhang Dawn J. Marshall Bing Chen Robert Parks Andrew Foulger Frederick Jaeger Michele Donathan Mira Bilska Elin S. Gray Salim S. Abdool Karim Thomas B. Kepler John Whitesides David Montefiori M. Anthony Moody Hua-Xin Liao Barton F. Haynes

Broadly neutralizing antibodies are not commonly produced in HIV-1 infected individuals nor by experimental HIV-1 vaccines. When these antibodies do occur, it is important to be able to isolate and characterize them to provide clues for vaccine design. CAP206 is a South African subtype C HIV-1-infected individual previously shown to have broadly neutralizing plasma antibodies targeting the enve...

2003
C. F. Ebenbichler N. M. Thielens R. Vornhagen P. Marschang G. J. Arlaud M. P. Dierich

Human immunodeficiency virus type 1 (HIV-1), in contrast to animal retroviruses such as murine leukemia virus, is not lysed by human complement. Nevertheless, HIV-1 activates complement via the classical pathway independent of antibody, and C3b deposition facilitates infection of complement receptor-bearing cells. Using gel exclusion chromatography on Sephacryl S-1000, purified virions were fou...

2013
Charles M. Gabrys Wei Qiang Yan Sun Li Xie Scott D. Schmick David P. Weliky

Fusion of the human immunodeficiency virus (HIV) membrane and the host cell membrane is an initial step of infection of the host cell. Fusion is catalyzed by gp41, which is an integral membrane protein of HIV. The fusion peptide (FP) is the ∼25 N-terminal residues of gp41 and is a domain of gp41 that plays a key role in fusion catalysis likely through interaction with the host cell membrane. Mu...

Journal: :Cell 1999
Joseph G Sodroski

transmembrane envelope glycoproteins. Most of the exJoseph G. Sodroski Department of Cancer Immunology and AIDS posed surface of the assembled trimer is composed of Dana-Farber Cancer Institute elements of gp120, with the gp41 ectodomain largely Department of Pathology buried in the interior of the complex (Figure 1, left panel). Harvard Medical School and The gp120 glycoprotein binds the host ...

Journal: :Journal of virology 2015
Suefen Kwa Shanmugalakshmi Sadagopal Xiaoying Shen Jung Joo Hong Sailaja Gangadhara Rahul Basu Blandine Victor Smita S Iyer Celia C LaBranche David C Montefiori Georgia D Tomaras Francois Villinger Bernard Moss Pamela A Kozlowski Rama Rao Amara

Here, we show that a CD40L-adjuvanted DNA/modified vaccinia virus Ankara (MVA) simian immunodeficiency virus (SIV) vaccine enhances protection against a pathogenic neutralization-resistant mucosal SIV infection, improves long-term viral control, and prevents AIDS. Analyses of serum IgG antibodies to linear peptides of SIV Env revealed a strong response to V2, with targeting of fewer epitopes in...

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