Fluorescently labelled nanoparticles are routinely used in Correlative Light Electron Microscopy (CLEM) to combine the capabilities of two separate microscope platforms: fluorescent light microscopy (LM) and electron microscopy (EM). The inherent assumption is that the fluorescent label observed under LM colocalises well with the electron dense nanoparticle observed in EM. Herein we show, by co...

The physical and antibacterial properties of ophthalmic lenses fabricated by copolymerization with hydrogel monomers using two types graphene were measured, their usability as contact lens materials was analyzed. For polymerization, silicone monomers, including SID-OH, 3-(methacryloxy)propyl tris(trimethylsiloxy)silane, decamethylcyclopentasiloxane, used, N,N-dimethylacetamide, ethylene glycol ...

Extinction coefficients of gold nanoparticles with core size ranging from approximately 4 to 40 nm were determined by high resolution transmission electron microscopy analysis and UV-vis absorption spectroscopic measurement. Three different types of gold nanoparticles were prepared and studied: citrate-stabilized nanoparticles in five different sizes; oleylamide-protected gold nanoparticles wit...

Inexpensive and rapid bacterial detection and antimicrobial susceptibility assays are desperately needed, as the determination of which antibiotic to use and its administration at effective dosages is critical. Currently, the assessment of antimicrobial susceptibility takes 24 to 48 hours. Hence, we have developed a nanoparticle-based antimicrobial susceptibility assay, utilizing the Concanaval...

Gold nanoparticles were synthesized by chemical reduction of HAuCl4 inside the pores of a polycarbonate-based membrane followed by dissolving the membrane in dichloromethane and further sonication. Sonication time as the main affecting factor on the nanoparticle size was investigated. The characterization by transmission electron microscopy showed the formation of gold nanoparticles with diamet...

Graphene nanoparticle dispersions show immense potential as multifunctional agents for in vivo biomedical applications. Herein, we follow regulatory guidelines for pharmaceuticals that recommend safety pharmacology assessment at least 10-100 times higher than the projected therapeutic dose, and present comprehensive single dose response, expanded acute toxicology, toxicokinetics, and respirator...

In this paper, amplification in dose due to the presence of gold nanoparticles was quantified using Monte Carlo N-Particle (MCNP) simulation. Simulation models included the irradiation of a 30 cm × 30 cm × 15 cm pure-water—volume phantom with a beam of 1.17 MeV and 1.33 MeV Cobalt spectral emissions as source. Planar and concentric water panel detectors placed at a distance of intervals of 0.1 ...

The present study assessed different methods of administering gold nanoparticles (GNPs) using different formulations to determine which of the methods achieved optimal radiosensitization. Cells from the B16F10 mouse melanoma cell line were implanted in the femoral area of mice, assigned to one of the eight following groups: i) Control; ii) intravenous (IV) injection of polyethylene glycol (PEG)...

metal nanoshells consists of a dielectric core surrounded by a thin noble metal shell, possess unique optical properties that render nanoshells attractive for use in different technologies. this paper reports a facile method for growth of small gold nanoparticles on the functionalized surface of larger silica nanoparticles. mono-dispersed silica particles and gold nanoparticles were prepared by...

Once nanoparticles enter a biological system, it is known that their surface is instantly covered by the biomolecules present with preference to proteins. This protein corona has been a subject of numerous studies in order to reveal its composition. Besides that, growing interest exists in its quantitative determination in order to gain a deeper insight into the nature of these nanoparticle-pro...