Alkaline hydrolysis of the crude resin glycoside fraction of the leaves, stems, and roots of Calystegia soldanella ROEM. et SCHULT. (Convolvulaceae) gave four new glycosidic acids, named calysolic acids A, B, C, and D, along with one known glycosidic acid, soldanellic acid B, and three organic acids, 2S-methylbutyric, tiglic, and 2S,3S-nilic acids. The structures of the new glycosidic acids wer...

Hydroxyl radical (·OH) is a key component that leads to the cleavage of glycosidic bond in process chitosan (CS) degradation by hydrodynamic cavitation (HC). In this paper, methylene blue (MB) was selected as trapping agent ·OH and yield an impact-jet hydraulic cavitator investigated. The results showed intensity number passes (N) were two main factors affecting ·OH. A smaller (Cv) or larger N ...

Maltogenic amylase (Mag1) is a potent enzyme that hydrolyzes the glycosidic bond of polysaccharides to produce malto-oligosaccharides (MOS). However, Mag1 has poor stability and reusability, leading inefficient MOS production. Enzyme immobilization promising method solve problem. Entrapment encapsulation technique was used in this study immobilize because high biocompatibility prevention degrad...

Alkylpurine glycosylase D (AlkD) exhibits a unique base excision strategy. Instead of interacting directly with the lesion, the enzyme engages the non-lesion DNA strand. AlkD induces flipping of the alkylated and opposing base accompanied by DNA stack compression. Since this strategy leaves the alkylated base solvent exposed, the means to achieve enzymatic cleavage had remained unclear. We dete...

The three-dimensional structure of a cyclic enterobacterial common antigen (ECA) having four trisaccharide repeating units has been investigated by NMR spectroscopy and molecular dynamics simulations. Three different NMR parameters were determined: (a) (1)H,(1)H cross-relaxation rates from NOE experiments were used for determination of proton-proton distances; (b) trans-glycosidic (3)J(C,H) sca...

Molecular dynamics (MD) simulations were used to study the stability and solvation of amylose and cellulose fragments. The recently developed gromos carbohydrate force field was further tested by simulating maltose, cellobiose, and maltoheptaose. The MD simulations reproduced fairly well the favorable conformations of disaccharides defined by the torsional angles related with the glycosidic bon...