We produced insertion mutants of herpes simplex virus (HSV) that contain two functional copies of genes encoding different forms of glycoprotein D (gD). These viruses have the gene for HSV type 2 (HSV-2) gD at the normal locus and the gene for HSV-1 gD inserted into the thymidine kinase locus. Results of immunoprecipitation experiments done with monoclonal antibodies revealed that both gD genes...

conclusions the comprehensive analysis of a protein with important roles has never been easy, and in case of e2 envelope glycoprotein of hgv, there is no much data on its molecular and immunological features, clinical significance and its pathogenic potential in hepatitis or any other gbv-c related diseases. so, results of the present study may explain some structural, physiological and immunol...

conclusions verapamil and rifampin were found specific and effective against p-gp expression in hcc. in conclusion, treatment efficacy of most anticancer drugs is increased in combination with verapamil and rifampin against most advanced hcc. objectives the aim of this study was to elucidate the effect of potent carcinogen nitrosamine with and without verapamil and rifampin drugs on p-gp expres...

Many hemopoietic cell lines were examined for their ability to adhere to culture dishes coated with extracellular matrix proteins. Adhesion assay was performed with murine and human leukemic cell lines representative of different stages of differentiation along both erythroid and myeloid lineages. All the hemopoietic cell lines tested adhered to fibronectin but not to laminin, types I, III, and...

214 words Introduction; 488 words Discussion; 931 words d) Abbreviations: ADx, adrenalectomized; DBP, D-site biding protein; E4BP4, E4 promoter binding protein-4; HLF, hepatic leukemia factor; P-gp, P-glycoprotein; ZT, zeitgeber time e) Recommended section: Metabolism, Transport and Pharmacogenomics This article has not been copyedited and formatted. The final version may differ from this versi...

Footpad adhesion sites pinch off from the rest of the cell surface during EGTA-mediated detachment of normal or virus-transformed murine cells from their tissue culture substrates. In these studies, highly purified trypsin and testicullar hyaluronidase were used to investigate the selective destruction or solubilization of proteins and polysaccharides in this substrate-attached material (SAM). ...

Hawthorn, an herbal supplement, is currently being evaluated for the treatment of heart failure. The flavonoid components of hawthorn may be responsible for hawthorn's beneficial effects in the treatment of heart failure. However, these components may also affect P-glycoprotein function and cause interactions with drugs that are P-glycoprotein substrates, such as digoxin, which is also used to ...

The relevant background information about the glycoprotein, enzymatic digestion, and the new matrix-assisted laser desorption/ionization time-of flight mass spectrometry (MALDITOF MS) ionization technique is reviewed. This method allows the detection of a complex mixture over a wide mass range (500-200,000 Da) using picomole to femtomole amounts of sample. Therefore, it is now possible to deter...

1130, page 191A.45. Dagan R, Melamed R, Abramson O, Piglansky L, Greenberg D, Mendelman P, et al. Effect ofheptavalent pneumococcal-OMPC conjugate vaccine on nasopharyngeal carriage when administeredduring the 2nd year of life. Presented at the Annual Meeting of the American Pediatric Society/Society forPediatric Research, San Diego, CA, 1995; Abstract #1020, page 172A.46. S...