A glucose-responsive controlled-release system based on the competitive combination between glucose oxidase, glucosamine and glucose has been described, which exhibits perfect controlled release properties and high selectivity for glucose over other monosaccharides. This paved the way for a new generation of stimuli-responsive delivery systems.

An economical, very sensitive and precise automated method for the determination of plasma glucose by glucose oxidase is presented. At 60 determinations per hour the method required 0.05 ml. of plasma and can conveniently determine glucose levels in the range of 0 to 500 mg/100 ml. Recovery experiments gave mean yields of 99% and, for 100 specimens analysed in duplicate, the precision was 1.24 ...

This report describes an interlaboratory comparison of enzymatic serum glucose methods as currently applied in several instrumental adaptations. Four spectrophotometric, hexokinase-based methods used with the Du Pont "aca," the Abbott "Bichromatic Analyzer," the Aminco "Rotochem," and the Technicon "AutoAnalyzer ll" were compared with glucose oxidase-based methods as used with the Beckman "Gluc...

An electrochemical response of glucose and lactate biosensors which were prepared by coating a platinum electrode with a thin film composed of concanavalin A and mannose-labeled glucose oxidase (GOx) or lactate oxidase (LOx) was evaluated in the presence of ferrocene derivatives as electron mediator. Both glucose and lactate biosensors showed catalytic current to glucose and lactate, respective...

Intact human neutrophils, incubated with the soluble stimulant phorbol myristate acetate, discharge lysosomal components, generate oxygen metabolites, and transform exogenous 6-keto-prostaglandin F1 alpha, prostaglandin E2, and prostaglandin F2 alpha as assessed by thin layer radiochromatography. Neutrophils alone were incapable of transforming the prostaglandins. The addition of catalase or th...

The monoolein-based liquid crystalline cubic phase was used as the matrix to incorporate redox enzymes--glucose (GOx), pyranose (PyOx) oxidases and laccase. Thin layer of the cubic phase embedding GOx or PyOx activated glucose oxidation in the presence and absence of appropriate mediators. The electrodes exhibited unchanged voltammetric response to glucose for not less than six days. The potent...

The coupling of the quinoprotein glucose dehydrogenase to the electron transport chain has been investigated in Acinetobacter calcoaceticus. No evidence was obtained to support a previous suggestion that the soluble form of the dehydrogenase and the soluble cytochrome b associated with it are involved in the oxidation of glucose. Analysis of cytochrome content, and of reduction of cytochromes i...

Abstract The synthesis of the emeraldine salt form polyaniline (PANI-ES) from aniline with Aspergillus sp. glucose oxidase (GOD), d -glucose, dissolved O 2 , and horseradish peroxidase isoenzyme C (HRPC) in presence large unilamellar vesicles AOT (sodium bis-(2-ethylhexyl)sulfosuccinate) as templates at pH = 4.3 T ~ 25 °C was investigated a systematic way. In this cascade reaction mixture, oxid...

The glucose-sensitive drug delivery systems based on glucose oxidase (GOD), which exhibit highly promising applications in diabetes therapy, have attracted much more interest in recent years. The self-regulated drug delivery systems regulate drug release by glucose concentration automatically and continuously to control the blood glucose level (BGL) in normoglycemic state. This review covers th...

A glucoseoxidase-peroxidasemethod for continuous automated monitoring of blood glucosehas been developed.The responseis linear over the range 0-800 mg/100 ml. Sensitivity can be maintained for 24 hr or longer and can be restored by rinsing the analytic system with sulfuric acid to permit studies of longer than 48 hr in duration. A precisionof ± 1% can be maintained between rinsesfor samplescont...