Samples (2ml) of a 605g,,. supernatant were applied to continuous linear gradients of 0.74-2.45 M-sucrose in 16 ml, on a cushion of 2ml of 2.45 M-sucrose. Centrifugation was at 4°C in a 3 x25ml swing-out rotor, and the integrated field-time was 562000g-min at ray. 9.39cm. Fractions (1 ml) were collected by displacement with 2.45~-sucrose. The letters A-D indicate the positions of the four fract...

A method was described for the partial purification of beta galactosidase and beta glucosaminidase from Clostridium tertium culture supernatants. Treatment of erythrocytes with preparations containing both enzymes decreases their ability to react with anti-I cold agglutinins, and with Type XIV antipneumococcal horse serum. Erythrocytes of blood group A(1) are altered more rapidly and extensivel...

Acute cardiorenal syndrome (CRS) corresponds to an association of acute heart failure and a worsening of renal function. The detection of acute kidney injury (AKI) unfortunately occurs at a late stage of CRS, leading to an increased mortality of the patients. In this review, we described the pathophysiology of CRS and discussed the potential interest of biochemical biomarkers (namely creatinine...

The rabbit corneal endothelium phagocytized polystyrene spheres 0.5 Mm in diameter. After phagocytizing spheres, the endothelium degenerated, and lost from the Descemet's membrane. Lysosomal enzyme activities of the endotheliumDescemet's membrane complex, such as acid phosphatase, /?-glucuronidase and N-acetyl-/?-D-glucosaminidase, were assayed and the total activities per microgram DNA were al...

We describe a kinetic, fluorometric assay for urinary Nacetyl-j3-glucosaminidase (EC 3.2.1.30) by measurement of the release of 4-methylumbelliferone (7-hydroxy-4-methylcoumarin). The method is simple, involving only a single reagent, and is applicable to the assay of other enzymes that hydrolyze similar fluorescent-labeled substrates. The enzyme distribution in human tissues and fluids is desc...

Land-use change and climatic variability are significant drivers for the loss of ecosystem services soil quality in prairie pothole region (PPR) wetland systems. induced changes groundwater table salinity may influence biogeochemical processes facilitated by extracellular enzymes (EEs) involved organic matter (SOM) decomposition. The effects changing on β-glucosidase (BG), N-acetyl glucosaminid...

Acute renal failure (ARF) secondary to ischemic injury remains a common and potentially devastating problem. A transcriptome-wide interrogation strategy was used to identify renal genes that are induced very early after renal ischemia, whose protein products might serve as novel biomarkers for ARF. Seven genes that are upregulated >10-fold were identified, one of which (Cyr61) has recently been...

We investigated the effects of uninephrectomy (UNX) in 6-week-old male and female rats on blood pressure (BP), renal sodium handling, salt sensitivity, oxidative stress, and renal injury over 18 months postsurgery, studying control sham-operated and UNX-operated rats at 6, 12, and 18 months postsurgery, evaluating their renal sodium handling, BP, urinary isoprostanes, N-acetyl-β-D-glucosaminida...