Fluorochrome staining to detect malaria parasites in bloodfilms is more sensitive, is easier to do, and is less time-consuming than Giemsa staining. However, standard epi-illuminated, mercury vapour, fluorescence microscopes are expensive, especially for tropical countries where malaria is endemic. Fluorescence microscopy with a standard light microscope and a new interference filter specially ...

A 50-year-old woman with a right cerebellopontine-angle schwannoma developed a chain of complications after multiple surgeries for tumor resection including intracerebral and subarachnoid hemorrhage, hydrocephalus, and fungal meningitis with subarachnoid pyogenic loculations. After 2 months of routine monitoring, cerebrospinal fluid (CSF) examination revealed increased red blood cells (RBCs), n...

Heterochromatin comprises a fraction of the genome usually with highly repeated DNA sequences and lacks of functional genes. This region can be revealed by using Giemsa C-banding, fluorochrome staining and cytomolecular tools. Some plant species are of particular interest through having a special type of heterochromatin denominated the cold-sensitive region (CSR). Independent of other chromosom...

Immunopathological investigations of the small round cells infiltrating into the tissues of human renal allografts and also those in urinary sediments were performed, using anti-human T-lymphocyte serum (AHTS). AHTS made from ALS is highly specific for T cells, but not for B cells. Tissues obtained by needle biopsy were examined by immunofluorescent staining and conventional histopathological m...

AIM To prepare a monoclonal antibody (EB9) against the trophozoite form of Pneumocystis carinii and to test its efficacy for detecting infection with this organism. METHOD The sensitivity and specificity of the EB9 antibody were assessed by comparing it with other conventional stains (Papanicolaou, Giemsa and Grocott) and 3F6 antibody in 33 bronchoalveolar lavage specimens from HIV positive p...

Sixty two patients diagnosed as having adult chlamydial ophthalmia were treated with oral doxycycline and roxithromycin in association and tetracycline eye wash for 2 weeks. Chlamydial ophthalmia was diagnosed by laboratory detection of the micro-organism in ocular specimens using direct immunofluorescent monoclonal antibody staining for Chlamydia trachomatis, chlamydial culture in cycloheximid...

The onset of X-chromosome inactivation was investigated cytologically in postimplantation female mouse embryos of age 5£, 6\ and 1\ days post-coitum (d.p.c.) and in the isolated epiblasts of 6 d.p.c. embryos before primitive streak formation using a heat/hypotonic technique to reveal the inactive X chromosome by differentially dark staining with Giemsa. The results indicate that X inactivation ...

The organization and replication of intranucleolar DNA in polytene cells of Drosophila nasuta have been examined. Normal Giemsa and fluorescent (Hoechst 33258 and quinacrine mustard) staining reveal that in D. nasuta polytene cells, the intranucleolar DNA is organized into condensed and disperse forms and very often shows connexion to the alpha heterochromatin of the chromocentre. Results of [3...

on a conventional ultramicrotome. Semi-thin sections on grids were stained with the Giemsa method and methylene blue-azure II-basic fuchsin. Staining without removal of the embedding matrix was sharp and brilliant. These stained sections were observed under a light microscope. For electron microscopy, they were doubly stained with uranyl acetate and lead salt. Identical sites on such sections c...

Comparative cytogenetic studies are very useful in the characterization of different accessions same plant. The aimed this study was search for intraspecific karyotypic variability three species Passiflora genus using chromosomal conventional techniques (Giemsa staining and Fluorochromes base-specific) molecular technique as Fluorescent Situ Hybridization (FISH). diploid character observed all ...