The study on utilization of carrot pomace powder (CPP) and germinated chickpea flour (GCF) in biscuits was undertaken to upgrade the nutritional quality and assess the acceptability. The biscuits were prepared from composite flours by incorporating 5, 8 and 10 parts of germinated chickpea flour and similar corresponding parts of carrot pomace powder in to wheat flour. The biscuits were analyzed...

In recent years, nutraceuticals attracted a great amount of attention in the biomedical research due to their significant contribution as natural agents for prevention of various health issues. Ethanolic extracts from the ungerminated and germinated seeds of Lupinus albus L. and Lupinus angustifolius L. were analyzed for the content in isoflavones (genistein) and cinnamic acid derivatives. Addi...

germinated within 24 hours after prior exposure either to blue light or when maintained under complete darkness. However, far-red light augmented the germination of seeds pretreated with 6-benzylaminopurine so that nearly all such seeds germinated within 24 hours after treatment (table II). Far-red light also augmented the rate of germination of seed pretreated with a synergistic mixture of 6-b...

Reactive Oxygen Species (ROS) are compounds derived from oxygen with important implications in biological processes plants, some of them related to reproduction. Among ROS, superoxide is the primary oxidant, since an array other ROS eventually this anion. Therefore, analysis molecular systems able generate molecule and cellular compartmentalization these events paramount importance. We have use...

Bacterial spores of various Bacillus species are impermeable or exhibit low permeability to many compounds that readily penetrate germinated spores, including methylamine. We now show that a lipid probe in the inner membrane of dormant spores of Bacillus megaterium and Bacillus subtilis is largely immobile, as measured by fluorescence redistribution after photobleaching, but becomes free to dif...

An efficient in vitro protocol has been established for somatic embryogenesis and plantlet conversion of Korean wild ginseng (Panax ginseng Meyer). Wild-type and mutant adventitious roots derived from the ginseng produced calluses on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid and 0.3 mg/L kinetin; 53.3% of the explants formed callus. Embryogenic ca...

Recent studies have suggested that dietary fiber exerts a therapeutic effect on inflammatory bowel disease patients. The aim of this study was to evaluate the effects of a dietary fiber, germinated barley foodstuff (GBF), derived from the aleurone and scutellum fraction of germinated barley against dextran sulfate sodium (DSS)-induced experimental colitis in rats. Sprague-Dawley rats were fed a...

Germinated seed from cereal crops including barley (Hordeum vulgare L.) is an important tissue to extract RNA and analyze expression levels of genes that control aspects of germination. These tissues are rich in polysaccharides and most methods for RNA extraction are not suitable to handle the excess polysaccharides. Here, we compare the current methods for RNA extraction applicable to germinat...

The aims of this study were to evaluate the effects of light and temperature on seed germination and the effect of light on seedling emergence of Clausena excavata, an invasive exotic species. Seeds were incubated at constant temperatures (10 to 40 ºC), under continuous white light and darkness. Seedling emergence was evaluated under full sun and canopy shade. There was no significant differenc...

The exudate of fully germinated spores of Clostridium perfringens was found to contain a large amount of a spore lytic enzyme which acted directly on alkali-treated spores of the organism to cause germination. Although no detectable amount of the enzyme was found in dormant spores during germination in a KCl medium, the enzyme was produced rapidly and released into the medium. The optimal condi...