Sumoylation during genotoxic stress regulates the composition of DNA repair complexes. The yeast metalloprotease Wss1 clears chromatin-bound sumoylated proteins. Wss1 and its mammalian analog, DVC1/Spartan, belong to minigluzincins family of proteases. Wss1 proteolytic activity is regulated by a cysteine switch mechanism activated by chemical stress and/or DNA binding. Wss1 is required for cell...

In the present study the effect of Centella asiatica L. extract was studied against the genotoxic doses of ethinylestradiol on human lymphocytes culture. The different doses of C. asiatica L. extract i.e. 1.075×10 -4 , 2.125×10 -4 and 3.15×10 -4 g/ml were treated separately with 5 μM of ethinylestradiol. Similar treatments were given with 10 μM of ethinylestradiol. The treatments result in a si...

Environmental pollution is a complex issue because of the diversity of anthropogenic agents, both chemical and physical, that have been detected and catalogued. The consequences to biota from exposure to genotoxic agents present an additional problem because of the potential for these agents to produce adverse change at the cellular and organismal levels. Past studies in genetic toxicology at t...

The prion protein (PrP) is highly conserved and ubiquitously expressed, suggesting that it plays an important physiological function. However, despite decades of investigation, this role remains elusive. Here, by using animal and cellular models, we unveil a key role of PrP in the DNA damage response. Exposure of neurons to a genotoxic stress activates PRNP transcription leading to an increased...

BACKGROUND AND OBJECTIVES: The eutrophication process is increased by anthropogenic or aquaculture facilities in marine ecosystems. DNA damage biomarkers for fish species detect genotoxic parameters for ecological risk assessment. The aim of the present study was to determine genotoxic potential induced by marine cage culture in Iskenderun Bay on gilthead sea bream (Sparus ...

(1) Background: Desalination is a developing industry that keeps expanding, nowadays counting >15,000 infrastructures worldwide. A byproduct of the desalination process concentrated brine, further containing operational chemicals, including antiscalants and coagulants. Yet, potential genotoxic impacts inclusive brine are inadequately studied. (2) Methods: In vitro in vivo assays were used to...

We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wa...

The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffe...

Formaldehyde (FA) is known to be genotoxic and mutagenic in proliferating mammalian cells in vitro. The present study was performed to further characterize its genotoxic potential in the V79 Chinese hamster cell line. The induction of DNA strand breaks and DNA-protein cross-links (DPXs) was measured by the comet assay in relationship to the induction of sister chromatid exchanges (SCEs) and mic...

In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aq...