Sporadic Alzheimer’s disease (AD) is a complex genetic disease, and the leading cause of dementia worldwide. Over past 3 decades, extensive pioneering research has discovered more than 70 common rare risk variants. These discoveries have contributed massively to our understanding pathogenesis AD but approximately half heritability for remains unaccounted for. There are regions genome that not a...

Abstract While the cost and time for assembling a genome has drastically decreased, it still remains challenge to assemble highly contiguous genome. These challenges are rapidly being overcome by integration of long-read sequencing technologies. Here, we use improve contiguity threespine stickleback fish (Gasterosteus aculeatus) genome, prominent genetic model species. Using Pacific Biosciences...

To locate the genes controlling adaptability in bread wheat using AMMI (additive main effect and multiplicative interaction) model, twenty-one substitution lines derived from the parents Chinese Spring (recipient) and Chayan (donor) were used in a randomized complete block design with three replications in three different environments in the Agricultural College of Razi University, Kermanshah, ...

The success of the Human Genome Project (HGP) has provided a blueprint for the approximately 20,000 gene-encoded proteins potentially active in all of the hundreds of cell types that make up the human body. Yet we still have limited knowledge about a majority of the gene-encoded proteins which are the “building blocks of life” and “cellular machinery”. It is estimated that for nearly half of th...

Abstract Background Cytoplasmic male sterility (CMS) is a trait associated with non-functional pollen or anthers, caused by the interaction between mitochondrial and nuclear genes. Findings A Tadukan-type CMS line (TAA) restorer (TAR) were obtained successive backcrossing Oryza sativa cultivars Tadukan (a cytoplasmic donor) Taichung 65 recurrent parent). Using Illumina HiSeq, we determined whol...

Extracellular vesicles (EVs) play crucial role in mediating intercellular communication. Small RNA is an important component EVs. However, the proportion of small sequencing (smRNA-seq) reads EVs mapped to human genome much lower than that cells, suggesting existence many nonhuman sequences there no systematic study on EV fragments unmapped genome. Herein, using smRNA-seq data, landscape exogen...

The reported genome-walking methods still suffer from some deficiencies, such as cumbersome experimental steps, short target amplicon, or deep background. Here, a simple and practical fork PCR was proposed for genome-walking. employs primer set of three random oligomers to implement walking task. In primary PCR, the low-stringency amplification cycle mediates binding places on genome, producing...

Their small sizes have meant that the Arabidopsis and rice genomes are the best-studied of all plant genomes. Although even closely related plant species can show large variations in genome size, extensive genome colinearity has been established at the genetic level and recently also at the gene level. This allows the transfer of information and resources assembled for rice and Arabidopsis to b...

This paper examines the model of network genomics pioneered in late 1980s and adopted European Commission-led Yeast Genome Sequencing Project (YGSP). It contrasted with burgeoning large-scale center being developed United States to sequence yeast genome, chiefly as a pilot for tackling human genome. We investigate operation connections two models by exploring co-authorship that captures differe...

Whole-genome sequencing technologies provide opportunities to further understand genetic variation among different varieties. Some related genes that are useful for the breeding process could be identified by technologies. In this study, two peach varieties, Green No. 9 and its bud mutant Daifei, were analyzed with whole-genome re-sequencing. Approximately 109 million total reads generated, whi...