Gene expression is the process by which information from a gene used in synthesis of functional product. With development techniques, many methods to analyze differentially expressed (DE) genes have emerged, especially downstream analysis approaches, such as limma, DESeq2, and edgeR. However, it unclear whether using different leads results. This article has compared results gained DESeq2 limma...

The effect of UV irradiation of bacteriophage T7 on in vivo early RNA synthesis has been studied by direct quantitation of the gene-specific RNA transcripts. The results show that the early region of phage T7 is transcribed from left to right as a single unit. Furthermore, gene inactivation, the UV sensitivity of synthesis of gene-specific RNA, and the UV sensitivity of synthesis of the corresp...

Coenzyme A (CoA) is a key molecule in cellular metabolism including the tricarboxylic acid cycle, fatty synthesis, amino synthesis and lipid metabolism. Moreover, CoA required for biological processes like protein post-translational modifications (PTMs) acylation. levels affect amount of histone acetylation thereby modulate gene expression. direct influence on other PTMs, CoAlation 4?-phosphopa...

introduction: beta thalassemia is the most common inherited bloody disorder, affecting synthesis of the beta globin chain of hemoglobin. the type of β-thalassemia mutation affects on the β-globin chain synthesis that appears as β ° ، β + and β ++ -thalassemia. the presence of xmni polymorphic site at the 5 َ region of the g γ-globin gene affects on the rate of g γ chain synthesis and in some con...

During herpes simplex virus infection, expression of the viral DNA polymerase (pol) gene is regulated temporally as an early (beta) gene and is additionally down-regulated at late times at the level of translation (D. R. Yager, A. I. Marcy, and D. M. Coen, J. Virol. 64:2217-2225, 1990). To examine the role of viral DNA synthesis in pol regulation, we studied pol expression during infections in ...

We show that the amber termination codon UAG can initiate protein synthesis in Escherichia coli. We mutated the initiation codon AUG of the chloramphenicol acetyltransferase (CAT) gene to UAG (CATam1) and translated mRNA derived from the mutant CAT gene in E. coli S-30 extracts. A full-length CAT polypeptide was synthesized in the presence of tRNA(fMetCUA), a mutant E. coli initiator tRNA which...

In Qβ RNA, sequestering the coat gene ribosome binding site in a putatively strong hairpin stem structure eliminated synthesis of coat protein and activated protein synthesis from the much weaker maturation gene initiation site, located 1300 nucleotides upstream. As the stability of a hairpin stem comprising the coat gene Shine-Dalgarno site was incrementally increased, there was a correspondin...

The synthesis of biotin, a vitamin required for many carboxylation reactions, is a variable trait in Saccharomyces cerevisiae. Many S. cerevisiae strains, including common laboratory strains, contain only a partial biotin synthesis pathway. We here report the identification of the first step necessary for the biotin synthesis pathway in S. cerevisiae. The biotin auxotroph strain S288c was able ...

diacylglycerol acyltransferase1 (dgat1) is a key enzyme in synthesis of triglycerides. a transition mutation resulting substitution of guanine by adenine in dgat1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. in this research, the polymorphism of the region spanning exon 3 to exon 17 of...

The requirement of the cdc2, cdc13 and cdc25 genes for meiosis in Schizosaccharomyces pombe was investigated using three different conditions to induce meiosis. These genes were known to be required for meiosis II. cdc13 and cdc25 are essential for meiosis I. The cdc2 gene, which is required for the initiation of both mitotic S-phase and M-phase, is essential for premeiotic DNA synthesis and me...