نتایج جستجو برای: geminivirus

تعداد نتایج: 589  

2012
Michael Aregger Basanta K. Borah Jonathan Seguin Rajendran Rajeswaran Ekaterina G. Gubaeva Anna S. Zvereva David Windels Franck Vazquez Todd Blevins Laurent Farinelli Mikhail M. Pooggin

In plants, RNA silencing-based antiviral defense is mediated by Dicer-like (DCL) proteins producing short interfering (si)RNAs. In Arabidopsis infected with the bipartite circular DNA geminivirus Cabbage leaf curl virus (CaLCuV), four distinct DCLs produce 21, 22 and 24 nt viral siRNAs. Using deep sequencing and blot hybridization, we found that viral siRNAs of each size-class densely cover the...

2011
A B S T R A C T Nirbhay Kushwaha Achuit K Singh Supriya Chakraborty

Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi – 110067, India E-mail: [email protected] 1 The detection and identification of viruses has been a challenge since the advent of the discipline of plant virology over a century ago. A great variety of methods have been developed that permit differentiation of viral pathogens. These methods, i...

Journal: :The Journal of general virology 2001
L C Berrie E P Rybicki M E Rey

Complete nucleotide sequences of the DNA-A (2800 nt) and DNA-B (2760 nt) components of a novel cassava-infecting begomovirus, South African cassava mosaic virus (SACMV), were determined and compared with various New World and Old World begomoviruses. SACMV is most closely related to East African cassava mosaic virus (EACMV) in both its DNA-A (85% with EACMV-MH and -MK) and -B (90% with EACMV-UG...

Journal: :Journal of virology 2003
Werner Preiss Holger Jeske

Geminiviruses package single-stranded circular DNA and replicate via double-stranded DNA intermediates. During the past decade, increasing evidence has led to the general acceptance that their replication follows a rolling-circle replication mechanism like bacteriophages with single-stranded DNA. In a recent study, we showed that this is also true for Abutilon mosaic geminivirus (AbMV), but tha...

Journal: :The Plant cell 2003
Roisin C McGarry Yoshimi D Barron Miguel F Carvalho Janet E Hill Daniel Gold Edwin Cheung W Lee Kraus Sondra G Lazarowitz

Protein acetylation is important in regulating DNA-templated processes specifically and protein-protein interactions more generally in eukaryotes. The geminivirus movement protein NSP is essential for virus movement, shuttling the viral DNA genome between the nucleus and the cytoplasm. We have identified a novel Arabidopsis protein, AtNSI, that interacts with NSP. AtNSI is highly conserved amon...

Journal: :The Plant journal : for cell and molecular biology 1998
T Kunik K Palanichelvam H Czosnek V Citovsky Y Gafni

The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly-transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem-associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investi...

Journal: :Virology 2005
Punjab Singh Malik Vikash Kumar Basavaraj Bagewadi Sunil K Mukherjee

In addition to their encapsidation function, viral coat proteins (CP) contribute to viral life cycle in many different ways. The CPs of the geminiviruses are responsible for intra- as well as inter-plant virus transmission and might determine the yield of viral DNA inside the infected tissues by either packaging the viral DNA or interfering with the viral replicative machinery. Since the cognat...

Journal: :Nucleic acids research 1992
K Saunders A Lucy J Stanley

The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is dem...

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