15 16 17 18 19 initiation and again after 360 s when the reaction is complete. The difference between the two readings closely apGDH, proximates the equilibriumabsorbance equlGDH, change. The rate determination uses an llbrium rate initial rate calculation. A set of seven g/L g/L absorbance readings is fit by linear re0 0 gression. The slope of the regression line 2.00 2.00 approximates the ini...

freshwater fish cirrhinus mrigala were exposed to lethal concentration (5 mg/l) for 1,2,3,4 days and sublethal concentration (1 mg/l) for 1,7,14,21 days of cypermethrin to examine the enzymes activity, in functionally three different tissues namely, gill, liver and muscle. activities of aspartate aminotransferase (aat), alanine aminotransferase (alat) and glutamate dehydrogenase (gdh) increased...

CONTEXT Diagnostic laboratory testing for Clostridium difficile infection has undergone considerable and rapid evolution during the last decade. The ideal detection method(s), which should exhibit high analytical and clinical sensitivity and specificity, remains undefined. OBJECTIVE We sought to evaluate the analytical and clinical performance characteristics of three methods for the laborato...

Chicory (Cichorium intybus), a deep rooted weed, grows in regions with temperate climates. Seasonal partitioning of compounds between the root and shoot results in fluctuations in the soluble carbohydrate, nitrate, amino acid, and protein pools within the roots. The activities of nitrate reductase (NR) (EC 1.6.6.1), glutamine synthetase (EC 6.3.1.2), NADH (EC 1.4.1.14), ferrodoxin glutamate syn...

The requirements for substrate binding in the quinoprotein glucose dehydrogenase (GDH) in the membranes of Escherichia coli are described, together with the changes in activity in a site-directed mutant in which His262 has been altered to a tyrosine residue (H262Y-GDH). The differences in catalytic efficiency between substrates are mainly related to differences in their affinity for the enzyme....

In this study, multi-wall carbon nanotubes (MWCNTs) were electrochemically modified with nitrogen and phosphorus species employed as platform to immobilize pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) for the fabrication of bioelectrodes detection. Depending on upper potential limit used during electrochemical modification MWCNTs, nature amount incorporated in material sur...

Polyaromatic hydrocarbons were electro-oxidized on CNT electrodes and studied towards their capacity to transfer electrons from the enzyme FAD-GDH electrode. A mixture of pyrene NHS gave high performing biocathodes.

UNLABELLED Soil-dwelling microbes solubilize mineral phosphates by secreting gluconic acid, which is produced from glucose by a periplasmic glucose dehydrogenase (GDH) that requires pyrroloquinoline quinone (PQQ) as a redox coenzyme. While GDH-dependent phosphate solubilization has been observed in numerous bacteria, little is known concerning the mechanism by which this process is regulated. H...

The Diffie-Hellman key-exchange protocol may naturally be extended to k > 2 parties. This gives rise to the generalized Diffie-Hellman assumption (GDH-Assumption). Naor and Reingold have recently shown an efficient construction of pseudo-random functions and proved its security based on the GDH-Assumption. In this note, we prove that breaking this assumption modulo a so called Blum-integer woul...