Gas chromatography-mass spectrometry and multivariate curve resolution were applied to the differential analysis of the volatile components in Agrimonia eupatoria specimens from different plant parts. After extracted with water distillation method, the volatile components in Agrimonia eupatoria from leaves and roots were detected by GC-MS. Then the qualitative and quantitative analysis of the v...

One of the uses of Gas Chromatography-Mass Spectrometry (GC-MS) is in the detection of pesticide residues in fruit and vegetables. In a high throughput laboratory there is the potential for sample swaps or mislabelling, as once a sample has been pre-processed to be injected into the GC-MS analyser, it is no longer distinguishable by eye. Possible consequences of such mistakes can be the destruc...

We describe a quantitative paperchromatographic method for assaying 3-methoxy-4-hydroxymandelic acid (VMA) in urine. VMA is extracted from acidified urine into ethyl acetate, separated from interfering amine, acid, and alcoholic metabolites by paper chromatography, and reacted with diazotized p-nitroaniline. The colored derivative is eluted and spectrophotometrically analyzed. Recoveries of rep...

Quantify proteins without stable isotopes Label-free quantifi cation Quantitative data are derived from the number of MS/ MS spectra per peptide (spectral counting) or the integrated peak area of the ion chromatogram of a specifi c peptide. Generally considered less accurate than isotope-based methods but does not require expensive labeling reagents; method of choice for large numbers of sample...

High-resolution anion-exchange chromatography of human urine can resolve more than 150 constituents that absorb ultraviolet light. Prefractionation of urine on Sephadex G.10 helped us identify the constituents and simplified the anion-exchange chromatogram. The material associated with the 13 peaks obtained by gel chromatography was pooled and concentrated into six fractions, which were subsequ...

A rapid determination of decabromodiphenyl ether (DeBDE) in polystyrene (PS) by thermal desorption (TD)-GC/MS was studied. The TD-GC/MS method using a pyrolysis-GC/MS system allowed the quick quantification of DeBDE in a waste TV back plate composing of a PS flame-retarded with polybrominated diphenyl ethers on the basis of the resulting chromatogram with a ca. 4% relative standard deviation wi...

Preparations from the parotid gland venom of the toad Bufo marinus have been analyzed by paper chromatography for the presence of adrenalin precursors. In addition to adrenalin itself, hydroxytyramine was identified and a second compound which appeared to be an adrenalin ester-perhaps lactyl-adrenalin. Two other compounds which appeared on the chromatogram could not be identified. Neither norad...

A blood sample from a 39-year-old female patient with newly diagnosed type 2 diabetes was assayed for hemoglobin (Hb) A1c on a Bio-Rad D10 HPLC platform. No peak for A1c was seen on the chromatogram (Fig. 1A), and the result was reported as 0%. A complete blood count was clinically significant for an Hb concentration of 10.9 g/dL (reference interval 12–15 g/dL) and a mean corpuscular volume of ...

Synthesis of indole derivatives was investigated and compared to the reaction of phenylhydrazine and ketones in the presence of the heterogeneous catalysts like kaolinite, montmorillonite K10 and Fe3O4 MNPs in ethanol under reflux conditions. After comparing the HPLC chromatogram of products it was compared and found that kaolin and montmorillonite K10 are better and more efficient candidate th...

Justicia adhatoda L. which belongs to the family of Acanthaceae contains alkaloids like vasicine, vasicinone and deoxyvasicine. These alkaloids give the plant its expectorant activity, antispasmodic, antiseptic and antihelmintic properties. Hence, in the present investigation attempts were made to isolate and characterise alkaloids from callus and suspension cultures of J. adhatoda. Alkaloids w...