Background: Ischemic stroke, as a health problem caused by the reduced blood supply to the brain, can lead to the neuronal death. The number of reliable therapies for stroke is limited. Mesenchymal stem cells (MSCs) exhibit therapeutic achievement. A major limitation of MSC application in cell therapy is the short survival span. MSCs affect target tissues through the secretion of many paracrine...

Thyroid cancer (TC) is the most prevalent malignant tumor in endocrine system. Serpin peptidase inhibitor clade E member 2 (SERPINE2) closely associated with metastasis. The aim of present study was to investigate whether SERPINE2 forms a feedback loop epidermal growth factor (EGF)/EGF receptor (EGFR) that regulates cellular processes human papillary thyroid carcinoma (TPC‑1) cells. Reverse tra...

Background: The objective of this study is to evaluate combined effect of Thymoquinone (The main active component of black seeds) with Tamoxifen drug on apoptosis of human breast cancer MCF-7 cells (Noninvasive human breast cancer cell line, estrogen receptor positive). Materials and Methods: The human breast cancer MCF- 7 cells were treated with Tamoxifen (2 μM) alone or in combination with Th...

p-nonylphenol (p-np) as a non ionic surfactant is used in many industries such as pesticides, cosmetics,detergents and so on. our previous study showed that the p-np causes reduction in viability of the rats bonemarrow mesenchymal stem cells (mscs). the aim of the present study was to investigate the mechanism of the cell death due to p-np exposure. the cells, after 3rd passage were treated wit...

in this study organotypic adult spinal cord slices were used to investigate whether caspases could participate in the apoptosis of motor neurons. the thoracic region of spinal cord was sliced using a tissue chopper and cultured in a medium for 6h. morphological and biochemical features of apoptosis were assessed by fluorescent staining and terminal deoxynucleotidyl nick end labeling (tunel) met...

Apoptosis plays a central role in the complex balance between invading pathogen and host defense. Depending on the pathogen, apoptosis of host cells may be beneficial or detrimental to survival. Studies using a clinically relevant intra-abdominal model of sepsis (peritonitis) have indicated that two types of cells, lymphocytes and gastrointestinal epithelial cells, undergo accelerated apoptosis...

PURPOSE To investigate whether apoptosis plays a notable role in degeneration of corneal endothelial cells in patients with Fuchs' dystrophy. METHODS Forty-seven corneal buttons from 41 patients with Fuchs' dystrophy were studied. Nucleus labeling, transmission electron microscopy (TEM), and TdT-dUTP terminal nick-end labeling (TUNEL) were used to detect apoptosis. TEM and TUNEL were performe...

This study determined whether retinal degeneration during diabetes includes retinal neural cell apoptosis. Image analysis of retinal sections from streptozotocin (STZ) diabetic rats after 7.5 months of STZ diabetes identified 22% and 14% reductions in the thickness of the inner plexiform and inner nuclear layers, respectively (P < 0. 001). The number of surviving ganglion cells was also reduced...

Cancer is one of the deadliest diseases against humans. To tackle this menace, humans have developed several high-technology therapies, such as chemotherapy, tomotherapy, targeted therapy, and antibody therapy. However, all these therapies have their own adverse side effects. Therefore, recent years have seen increased attention being given to the natural food for complementary therapy, which h...