We identified two genes related to fungicide resistance in Fusarium fujikuroi through random mutagenesis. Targeted gene deletions showed that survival factor 1 deletion resulted in higher sensitivity to fungicides, while deletion of the gene encoding F-box/WD-repeat protein increased resistance, suggesting that the genes affect fungicide resistance in different ways.

Expression in Pisum and Lathyrus. Sandhya Tewari1,3, Stuart M. Brown1,2 and Brian Fristensky1 1Department of Plant Science, University of Manitoba, Winnipeg, R3T 2N2, CANADA. Current address: 2Cell Biology Department, NYU Medical Center, 550 First Avenue, New York, NY 10016, U.S.A. 3Confederation of Indian Industry, 23 Institutional Area, Lodi Road, New Delhi 110 003 INDIA Corresponding author:...

Zearalenol was extracted from Fusarium-infected stems of corn from southern Italy. The toxin, which appeared as a single compound in various thin-layer chromatography systems, was resolved by high-pressure liquid chromatography into two components. A gas chromatography-mass spectrometry examination of a purified fraction confirmed the natural occurrence of zearalenol as a diastereomeric mixture...

We investigated the interaction between amphotericin B and azithromycin in vitro against 26 clinical isolates of Fusarium. Synergy was demonstrated in all isolates. Amphotericin B MICs were reduced from a mean of 1 mg/L when tested alone to a mean of 0.37 mg/L when tested in combination with azithromycin. Azithromycin demonstrated no activity against Fusarium when tested alone (MIC > 128 mg/L)....

In South Africa, the main causal organisms of Fusarium head blight (FHB) are Gibberella zeae (=Fusarium graminearum), F. culmorum and F. crookwellense. Fusarium graminearum and F. culmorum are associated with warmer regions, and F. crookwellense with cooler regions. Sporadic FHB outbreaks occur principally in the irrigation areas of the country. In favourable years significant damage is caused....

Fusarium is a type of mould capable of producing several diseases in cereals. Infection is a worldwide problem associated with yield losses and the accumulation of toxic secondary metabolites, mycotoxins, which are harmful to both humans and animals. F. graminearum, F. culmorum, F. avenaceum, F. poae, and F. tricinctum, including corresponding mycotoxins were quantified in wheat samples from 6 ...

Twenty-five isolates of Fusarium fujikuroi acquired from rice seeds and rice plants evidencing symptoms of Bakanae disease were evaluated to determine their mating types and characterize the formation of their sexual state. The mating types of the isolates were evaluated via multiplex PCR with the diagnostic primers of the mating-type (MAT) region: GFmat1a, GFmat1b, GFmat2c, and GFmat2d. Among ...

Rabbit antibody to cutinase-I, isolated from Fusarium solani f. pisi, was conjugated to ferritin. With this ferritin-conjugated antibody it was shown that germinating spores of this fungus excreted cutinase during the penetration of the host pisum sativum. This result constitutes the most specific and strongest evidence for an enzymic penetration of a plant cuticle by a pathogen during infection.

Eleven antifungal drugs were tested against representative isolates of the four phylogenetic clades of the Fusarium solani species complex obtained in a multilocus sequence analysis. They all showed very poor activity, with no differences among the clades. Amphotericin B was the most active drug.