STUDY QUESTION What are the attitudes towards different aspects of embryo donation among Swedish infertile couples who have surplus cryopreserved embryos? SUMMARY ANSWER Nearly three-quarters of infertile couples with surplus embryos were in favour of embryo donation. A majority of respondents were also in favour of embryos being donated for research. WHAT IS KNOWN ALREADY Currently, embryo...

Since the birth of the first baby as a result of in vitro fertilisation (IVF) in 1978, many clinics around the world have achieved pregnancies and births for their patients by using IVF and gamete intrafallopian transfer procedures. With the storage of excess embryos, multiple laparoscopies can be avoided; this has favoured the development of better cryopreservation techniques. In our clinic 8-...

Biological technology used for purposes of human reproduction has generated a number of issues involving contract law, genetic parenthood, and public policy. One such issue arises from disagreements about the fate of frozen embryos. This issue presents special challenges to the judicial process even though historically courts have had experience in applying reason to the most emotional of issue...

Morphological changes of cultured bovine blastocysts during hatching were observed using time-lapse videomicrography in order to investigate the patterns of the hatching process that occurred in the blastocysts and to determine whether the hatching patterns differed between blastocysts developed from fresh and cryopreserved embryos. Compacted morulae (CMs) were collected from superovulation-tre...

The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with surfactant and embryotrophic properties, such as recombinant albumin (RA) and hyaluronan (HA). The first part of the study was dedicated to define the ...

Background: The opportunity to completely ‘stop biological time’ using cryopreservation, as it is used in embryo provides for the selection and long-term storage of high-quality preimplantation embryos future usage. While this a beneficial medical procedure, has however posed some legal issues such status an embryo, ownership reproductive autonomy, posthumous reprodu...

Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...

Embryo cryopreservation is a routine procedure in assisted reproductive technologies. Although couples have been informed about all potential procedures, some of them face the dilemma of what to do with surplus frozen embryos. The purpose of this qualitative study was to evaluate the attitude of patients toward their surplus cryopreserved embryos. Fifty patients who had undergone successful IVF...

Transgenesis constitutes an important tool for pharmacological protein production and livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to produce egfp-expressing ovine embryos, using spermatozoa previously exposed to pCX-EGFP plasmid in two different sperm/DNA incubation treatments: "Lon...