Pendahuluan: Nanoemulsi adalah sistem emulsi transparan, terdiri dari campuran minyak dan air, serta molekul surfaktan untuk menstabilkannya. Ukuran partikel nanoemulsi berkisar antara 10-200 nm. Kandungan alkaloid, flavonoid, tannin, saponin eugenol pada daun kemangi bermanfaat sebagai antibakteri. Tujuan penelitian ini mengevaluasi stabilitas fisik formulasi ekstrak menggunakan tween 80 denga...

Physically cross-linked cryogels can be prepared using linear polyethyleneimine (L-PEI) and a freeze-thawing technique. L-PEI was synthesized by hydrolysis of poly(2-ethyl-2-oxazoline) under acidic conditions. Dissolution in deionized water achieved at 80 °C resulted transparent solution, leading to the formation an opaque gel upon freezing subsequent thawing. The exhibited reversibility, after...

Background: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to both semen and sperm after repeated freezing and thawing in oligozoospermic and normozoospermic samples ...

The aim of this study was to define a simple extender for freezing rabbit semen from selected males used to inseminate selected doses to obtain embryos for an embryo bank. Four experiments were carried out. In the first experiment, freezing extender was defined on the basis of the results of the post-thawing motility rate. Three factors and their interactions were studied: final concentration o...

The fertility (positive ultrasound scanning 60-75 days after insemination) of ram spermatozoa frozen in Tris- or zwitterion/glucose/egg yolk/glycerol-based diluents was examined during the natural breeding season (autumn). In Experiment 1 Tris-citrate, high glucose and Hepes-NaOH diluents were used and ewes (n = 266) were inseminated (induced oestrus) with 20 (uterus) or 180 (cervix) million mo...

The response of the drought-tolerant moss Tortula ruralis ([Hedw.] Gaertn., Meyer, Scherb.) to freezing and thawing at controlled rates has been studied. Slow freezing (at 3 C per hour to -30 C) of hydrated T. ruralis leads to only temporary, reversible changes in metabolism. These changes can be considered to result from desiccation due to extracellular ice formation. In contrast, rapid freezi...

Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30 cell separator and an Aminco Celltrifuge. The best results were achieved using the paediatric pheresis set on the Hemonetics model 30. Marrow was frozen in 120 ml aliquots in a programmed freezer with ...

Cryopreservation of human spermatozoa has been used since 1954 (1). After surgical treatment, chemotherapy, or radiotherapy, permanent sterility or transient infertility of up to 5 years can occur (2). Therefore, cryopreservation is often recommended to preserve future fertility. However, sperm survival and motility are known to significantly decrease after the freeze-thaw process. Since sperm ...

Triton X-100 treatment or freeze-thawing damages the membranes of sporulating or vegetative cells as seen by protein leakage from cells. A 40% increase in the specific [(14)C]benzylpenicillin-binding capacity of detergent-treated or frozen sporulating cells was observed. Neither freezing nor Triton X-100 treatment of vegetative cells produced a detectable effect on their [(14)C]benzylpenicillin...