Background: Cryobiology and in vitro maturation of oocytes are very important tools of reproductive technology. Oocyte plays an important role in fertility. The objective of this research is comparison study the effect of invitro maturation and IVF fertilization of cryopreserved oocyte with fresh oocyte in mice. Materials and Methods: For this purpose, after superovulation was performed oocytes...

This study was conducted to evaluate the activities of antioxidant enzyme (glutathione peroxidase (GSH-Px)) and lysosomal enzymes (alpha-glucopyranosidase (AGP) and beta-N-acetyl-glucosaminidase (BNAG)) of the longissimus dorsi (LD) muscle from Hanwoo (Korean cattle) in three freezing conditions. Following freezing at -20, -60, and -196℃ (liquid nitrogen), LD samples (48 h post-slaughter) were ...

BACKGROUND We investigated the encapsulation mechanism of enzymes into liposomes. The existing protocols to achieve high encapsulation efficiencies are basically optimized for chemically stable molecules. Enzymes, however, are fragile and encapsulation requires in addition the preservation of their functionality. Using acetylcholinesterase as a model, we found that most protocols lead to a rapi...

The relative effect of a freeze-thaw cycle on photosynthesis, respiration, and ion leakage of potato leaf tissue was examined in two potato species, Solanum acaule Bitt. and Solanum commersonii Dun. Photosynthesis was found to be much more sensitive to freezing stress than was respiration, and demonstrated more than a 60% inhibition before any impairment of respiratory function was observed. Ph...

Although biochar has been investigated extensively to stabilize soil heavy metals, there were limited investigation on the stabilization of metals by using under natural ageing processes alternate-cooling/heating. In this study, wheat-straw-biochar-treated samples contaminated with Pb(II) ions/PbO subjected 12 h-freeze/12 h-thaw cycles simulating alternate-cooling/heating process. After freeze-...

Based on the orthogonal test, optimized ratio of hybrid fiber fly ash concrete prepared with different volume admixtures polypropylene and polyacrylonitrile fiber, lengths, was obtained, its frost resistance evaluated in terms mass change, dynamic elastic modulus, compressive strength, splitting tensile strength before after water salt freezing. Then, damage mechanism discussed by combining por...

The ability of isolated Malpighian tubules from a freeze-tolerant insect, the New Zealand alpine weta (Hemideina maori), to withstand freezing was assessed by measuring post-freeze membrane potentials and rates of fluid secretion. The hemolymph of cold-acclimated Hemideina maori was found to contain relatively high concentrations of the cryoprotectants trehalose (>300 mmol l-1) and proline (41 ...

background: leptin and its receptor are present in spermatozoa; however, the role of leptin in sperm function is still controversial. our present study aimed at demonstrating the effect of cryopreservation on sperm dna fragmentation (dnaf) and investigating the possible effects of sperm capacitation techniques and leptin in vitro incubation on frozen-thawed sperm dnaf and oxidative stress. meth...