Male and female rats respond to a fearful experience in different ways, but this was not previously taken into account in research into psychiatric disorders.

Rat hearts were perfused for 15 min with the concentration of glucose indicated, with or without insulin (1 munit/ml). The tissue was freeze-clamped. Fructose 2,6-bisphosphate content and PFK-2 activity in 20% (w/v) polyethylene glycol fraction were measured as described by Rider & Hue (1984). Values are means ~ s . E . M . for the number of experiments shown in parentheses. LDH, Lactate dehydr...

The influence of aluminosilicates on the structure and drug release profiles of polyvinyl alcohol (PVA) – halloysite (HNT) hydrogels containing acetylsalicylic acid (ASA) as a model drug was monitored. The hydrogels were synthesized using a three cycle freeze – thawing procedure and were characterized by FTIR, XRD and SEM. The swelling degree and cytotoxicity were also determined. All hydrogels...

Immunogen: 3-Nitrotyrosine-KLH Clone: 3B13 Isotype: IgG1 Host animal: Mouse Format: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3mM sodium azide, pH 7.5 Ship Conditions: Ship at ambient temperature, freeze upon arrival Species Reactivity: Ubiquitous Applications: Antibody can be used for Western blotting (Suggested dilution 1:1000) and immunohistochemistry. Optimal concentrati...

Recently, increasing attention has been paid to lecithin/chitosan nanoparticles as colloidal drug delivery system showing the great potential to improve the permeation of encapsulated drug across various biological barriers [1, 2]. However, the overall poor stability of colloidal nanoparticles in an aqueous medium is a significant drawback in their development perspective. Therefore, the aim of...

Because procedures of handling and storage of body fluids affect numbers and composition of extracellular vesicles (EVs), standardization is important to ensure reliable and comparable measurements of EVs in a clinical environment. We aimed to develop standard protocols for handling and storage of human body fluids for EV analysis. Conditions such as centrifugation, single freeze-thaw cycle, ef...

In freeze-thaw lysates of MDCK cells infected with 32P-labeled influenza virus A/WSN in the presence of added RNase, acid-precipitable radioactivity diminished to about 50% of initial values within 90 min after a 1-h virus adsorption period. A similar preparation containing rimantadine at a concentration of 50 micrograms/ml exhibited only a 10% reduction in acid-precipitable radioactivity. Thes...

BACKGROUND Reduced cerebrospinal fluid (CSF) concentration of amyloid-β1-42 (Aβ1-42) reflects the presence of amyloidopathy in brains of subjects with Alzheimer's disease (AD). OBJECTIVE To qualify the use of Aβ1-42/Aβ1-40 for improvement of standard operating procedures (SOP) for measurement of CSF Aβ with a focus on CSF collection, storage, and analysis. METHODS Euroimmun ELISAs for CSF A...

freeze-thaw is one of the most powerful weathering agents that may cause a rapid change in the initial engineering properties of stones, and limit their durability. freeze-thaw induces stress over the pores’ surface of stones. consequently, stone durability is closely related to its engineering and physical properties. the purpose of this study is estimating the engineering properties of deteri...