The differentiation agent hexamethylene bisacetamide (HMBA) increased expression of transforming growth factor beta 1 (TGF beta 1) mRNA in HT29 colon carcinoma cells. The increase was evident after 24 h and was maintained at levels 4-5-fold the control levels for at least 5-13 days. No increase in expression of TGF beta 2 or TGF alpha mRNA was observed. Both TGF beta 1 and HMBA induced loss of ...

We apply our recently developed metadynamics method to the docking of ligands on flexible receptors in water solution. This method mimics the real dynamics of a ligand exiting or entering an enzyme and in so doing reconstructs the free energy surface. We apply it to four docking cases: beta-trypsin/benzamidine, beta-trypsin/chlorobenzamidine, immunoglobulin McPC-603/phosphocholine, and cyclin-d...

The addition of hydralazine (1-hydrazinophthalazine) to rat liver mitochondria metabolizing malate/glutamate causes formation of a carbon-centered free radical which was spin-trapped with phenyl-t-butylnitrone (PBN) or dimethylpyrrolidine-N-oxide (DMPO). The coupling constants of the spin-trapped free radical were AN = 16.1, AH beta = 4.6 G for PBN and AN = 15.9, AH beta = 18.9 G for DMPO-trapp...

Scale-free dynamics, with a power spectrum following P proportional to f(-beta), are an intrinsic feature of many complex processes in nature. In neural systems, scale-free activity is often neglected in electrophysiological research. Here, we investigate scale-free dynamics in human brain and show that it contains extensive nested frequencies, with the phase of lower frequencies modulating the...

The synthesis and dimerization of beta-chains during the formation of catalytically active beta-hexosaminidase B were studied in a cell-free system. beta-chain mRNA, transcribed from the cloned cDNA with SP6 polymerase, was translated in a rabbit reticulocyte protein-synthesizing system in the presence of dog pancreas microsomal membranes and oxidized glutathione. Under these conditions, the pr...

Cytotoxic T lymphocytes (CTL) can recognize antigenic peptides bound in a groove formed by the alpha 1 and alpha 2 domains of the heterodimeric major histocompatibility complex class I molecule. Proper assembly, transport, and stability of functional class I molecules is thought to require beta 2 microglobulin (beta 2m), the light chain of the class I heterodimer. We show here that the requirem...

We present experimental data providing evidence for the formation of transient ($\sim 20~\mu$s) plasmas that are simultaneously weakly magnetized (i.e., Hall magnetization parameter $\omega \tau > 1$) and dominated by thermal pressure ratio thermal-to-magnetic $\beta 1$). Particle collisional mean free paths an appreciable fraction overall system size. These formed via head-on merging two launc...

Abstract We study the intermediate inflation in mimetic Dirac–Born–Infeld model. By considering scale factor as $$a=a_{0}\exp (bt^{\beta })$$ a = 0 exp ( b t β</mml:mi...

Various gedanken experiments of quantum gravity phenomenology in search a complete theory near the Planck scale indicate modification Heisenberg uncertainty principle to generalized (GUP). This leads nontrivial contributions on Hamiltonian nonrelativistic particle moving an arbitrary potential. In this paper we study path integral representation potential using most general form GUP containing ...