This article describes the optimization of cultivation factor settings, that is the shaking rate and working volume in 50 mL spin tubes for a Chinese hamster ovary cell line expressing recombinant human alpha-erythropoietin, using a response D-optimal surface method. The main objectives of the research were, firstly, to determine a setting in which the product titer and product quality attribut...

the process of spatial distribution of urban services in order to provide equitable access to opportunities and reduced regional disparities, and earning the highest citizen satisfaction are among the main challenges facing urban management. this requires knowledge of the current status of spatial distribution of public services in the city, followed by optimal resource allocation under varying...

I study the influence of anisotropic spin exchange on a quantum phase transition in the Plaquette lattice driven by the purely quantum effect of singlet formation. I study the influence of i) a Dzyaloshinskii-Moriya exchange and ii) four spin exchange on the transition point by evaluating spin–spin correlations and the spin gap with exact diagonalization. The results point to a stabilization of...

Integration of sample preparation is a key issue in the development of point of care diagnostic devices. We address the integration of a magnetic-particle based DNA extraction from 200 μl of whole blood into a centrifugal-microfluidic LabDisk. Quality of LabDisk extracted DNA was compared to DNA extracted by commercial spin columns as golden standard with four independent extractions from one b...

Background and Objectives: Curcumin and exercise training are suggested to improve hepatic disorders. The aim of this study was to investigate effects of two weeks of swimming training and curcumin supplementation on platelet-derived growth factor (PDGF-BB) and transforming growth factor beta 1 (TGF-β1) gene expressions following ethanol consumption in Wistar male rats. Materials and Methods: ...

Dipolar truncation prevents accurate measurement of long-range internuclear distances between nuclei of the same spin species, e.g., within (13)C-(13)C spin pairs in uniformly (13)C-isotope-labeled proteins, using magic-angle spinning solid-state NMR spectroscopy. Accordingly, one of the richest sources of accurate structure information is at present not exploited fully, leaving the bulk part o...

Tricalcium phosphate (TCP)-based materials, such as ?-Ca3(PO4)2 doped with rare earth ions (RE), have shown applications biomaterials, lighting emitting scintillating in vivo imaging probes, and thermoluminescent dosimeters. Their properties are found to be dependent on the distribution of RE3+ Ca2+ sites that can controlled by pulsed electron paramagnetic resonance (EPR) spin echo envelop modu...