E. coli β-glucuronidase (GUS) activity assays are routinely used in fields such as plant molecular biology, applied microbiology and healthcare. Methods based on the optical detection of GUS using synthetic fluorogenic substrates are widely employed since they don't require expensive instrumentation and are easy to perform. In this study three fluorogenic substrates and their respective fluorop...

Introduction Many techniques of cellular and molecular biology require the ability to quantitate dsDNA in large numbers of samples at sensitivities that only require a small amount of the total sample. Isolation of plasmids from bacterial cultures, genomic DNA from mammalian cells, cDNA synthesis for library production, and quantitation of PCR products for diagnostic purposes all require the di...

Samplers are characterized at ECBC using the following methods: (1) monodisperse fluorescent/nonfluorescent PSL microspheres with fluorometric analysis or Counter analysis, (2) polydisperse solid aluminum oxide particles with APS analysis or Coulter Multisizer analysis, (3) fluorescent oleic acid particles with fluorometric analysis, and (4) bioparticles with Coulter Multisizer, culturing, ELIS...

In this brief review, gold nanoparticles conjugated with functional polymers are described from the viewpoint of application to sensing materials. The optical properties of gold nanoparticles, the synthesis of polymer-functionalized gold nanoparticles, and their analytical applications are discussed. Polymer-functionalized gold nanoparticles are categorized into two classes: biopolymer-conjugat...