abstractbackground : in order to quantify effects of ultrasound irradiation parameters under therapeutic condition, especially sonodynamic therapy, it is initially necessary to evaluate inertial cavitation activity in vitro conditions therefore, in this study, the effect of 1 mhz low level ultrasound based on °oh radicals generated by acoustic inertial cavitation in aqueous solution was monitor...

In this study, we investigated the effects of different hydrogen bond types on photophysical properties diethylamino hydroxybenzoyl hexyl benzoate (DHHB) by systematically exploring changes in spectral DHHB protic and aprotic solvents. The formation stability bonds were also studied, demonstrating that system mainly existed form intramolecular at low concentrations (≤5 μg/mL). these circumstanc...

Application of green fluorescent protein (GFP) as a molecular biosensor or reporter is limited by several factors especially its poor fluorescence at low pH conditions. There are conflicting data on this limitation, however, systematic studies to assess the importance of this factor for growing bacterial cultures are lacking. In this study, effect of low pH on GFP green and red fluorescence int...

Metal surfaces and nanostructures interact with fluorescent materials, enhancing or quenching the fluorescence intensity, modifying the fluorescent lifetime, and changing the emission frequency and linewidth. These interactions occur via several mechanisms, including radiationless energy transfer, electric field enhancement, and photonic mode density modification. The interactions display a str...

BACKGROUND Endoscopy capable of fluorescence observation provides histological information on gastrointestinal lesions. We explored the novel application of low pH-dependent fluorescent dyes for fluorescence observation of crypt structure and inflammatory cell infiltration in the colon. METHODS Low pH-dependent fluorescent dyes were applied to the colonic mucosa of normal mice for observation...

chitosan-graft-polyethylenimine (chi-g-pei) copolymer has been used for theimprovement of low transfection efficiency of chitosan. the present study aims to test thepulmonary toxicity and efficiency of chi-g-pei as an aerosol gene carrier. mice were exposedto aerosol containing green-fluorescent protein (gfp)-polyethylenimine (pei) or gfp-chig-pei complexes for 30 min during the development of ...

1 Yuhei Natori, MD Hidekazu Yoshizawa, MD Daiki Senda, MD Hiroshi Mizuno, MD Ayato Hayashi, MD Department of Plastic and Reconstructive Surgery Juntendo University School of Medicine Tokyo 113-8421, Japan Sir: N regeneration has been electrophysiologically, histomorphometrically, and immunocytochemically evaluated.1 However, these techniques only evaluate the regeneration state at specific time...

background and aims: rubella is predominantly a childhood disease that is endemic throughout the world and when rubella outbreaks occur, they are accompanied by birth defects following congenital rubella syndrome. immunity to rubella virus as a teratogenic agent has an important role for prevention of these serious congenital defects. lymphocyte proliferation assay is a way for investigation of...