BACKGROUND Enterohemorrhagic Escherichia coli (EHEC) cause diarrhea-associated hemolytic uremic syndrome (D+ HUS) worldwide, but no systematic study of EHEC as the causative agents of HUS was performed in the Czech Republic. We analyzed stools of all patients with D+ HUS in the Czech Republic between 1998 and 2012 for evidence of EHEC infection. We determined virulence profiles, phenotypes, ant...

nosocomial infections of multidrug-resistant pseudomonas aeruginosa (mdrpa) are a growing concern in hospitalized patients in burn centers. the aim of this study was to investigate the flagellin profiling and antibiotic susceptibility of p. aeruginosa isolated from burn wound infections. during 8 month study, 73 clinically p. aeruginosa isolates collected from patients hospitalized in burn ward...

SpiC is a virulence factor encoded within Salmonella pathogenicity island 2 (SPI-2). We have previously reported that infection of macrophages with Salmonella enterica serovar Typhimurium results in the SPI-2-dependent activation of the mitogen-activated protein kinase (MAPK) signalling pathways, leading to the expression of suppressor of cytokine signalling (SOCS)-3, which is involved in the i...

Lateral flow tests also known as Immunochromatography (IC) is an antigen-detection method conducted on a nitrocellulose membrane that can be completed in less than 20 min. IC has been used as an important rapid test for clinical diagnosis and surveillance of influenza viruses, but the IC sensitivity is relatively low (approximately 60%) and the limit of detection (LOD) is as low as 10³ pfu per ...

Bacterial flagellin is a potent host immune activator. Parys et al., 2021Parys K. Colaianni N.R. Lee H.-K. Hohmann U. Edelbacher N. Trgovcevic A. Blahovska Z. D. Mechtler Muhari-Portik al.Signatures of antagonistic pleiotropy in bacterial epitope.Cell Host Microbe. 2021; 29 (this issue): 620-634Google Scholar and 2021Colaianni H.-S. Conway J.M. Kim N.H. Mucyn T.S. Madalinski M. Law T.F. Jones C...

It is widely accepted that bacterial infection-mediated inflammation facilitates development of atherosclerosis by activating toll-like receptor (TLR) signaling system. We reasoned that NADPH oxidases (Nox), required for TLR-mediated inflammatory response, are involved in atherogenesis. Here, we show that the activation of Nox4 through TLR5 regulates the inflammation of the endothelium and in a...