A variety of fluorescent tracers and proteins were injected via severed aphid stylets into the sieve tubes of wheat (Triticum aestivum L.) grains to evaluate the dimensions of plasmodesmal channels involved in sieve element/companion cell (SE/CC) unloading and post-phloem transport. In the post-phloem pathway, where diffusion is the predominant mode of transport, the largest molecule to show mo...

The relative functional maturity of neonatal mouse spleen T- and B-cell populations was assessed by comparing the ability to respond to the thymic-independent antigen, DNP-Ficoll, or thymic-dependent SRBC by producing antibody in vitro. Although mouse spleen cells responded to DNP-Ficoll at an earlier age than they responded to SRBC or TNP-SRBC, the reason for the lag in the T-dependent respons...

A simple method for labeling human lymphocytes with Hg-197 is described. Lymphocytes were isolated from human blood and purified by the Ficoll-Hypaque method. Labeling was carried out by incubating viable cells suspended in saline with Hg-197 for different time intervals and at different temperatures. The labeling yield varied from 48% for 1 million cells to about 70% for 10 million cells after...

INTRODUCTION The aim of the study was to explore the distribution of healthy people with different anti-tumour ability. MATERIAL AND METHODS Leukocytes were separated by the Ficoll-Hypaque density gradient centrifugal method. Then they were mixed with A549, MCF-7 and Hela cells at different ratios. The survival rate for target cells was observed and counted by Fluoroskan. Immune function for ...

A PREPARATIVE METHOD FOR OBTAINING ENUCLEATED MAMMALIAN CELLS Michael H. Wigler and I. Bernard Weinstein Institute of Cancer Research and Departments of Medicine and Microbiology, College of Physicians and Surgeons Columbia University, New York, New York 10032 Received February 3, 1975 SUMMARY Mouse L cells can be enucleated in suspension by centrifugation in discontinuous Ficoll density gradie...