A 46 kDa heparin-binding protein which inhibits thymidine incorporation in cultures of fetal calf liver erythroid cells was isolated from fetal bovine serum by affinity chromatography on heparin-Sepharose, ion-exchange chromatography, gel filtration and reversed-phase h.p.l.c. The N-terminal sequence of the first 22 amino acids showed 81% identity with the published sequence of human apolipopro...

The study shows that multiaxial deformation (MAD) treatment leads to grain refinement in magnesium alloy WE43. Compared the initial state, MAD-processed exhibited smoother biocorrosion dynamics a fetal bovine serum and complete cell growth medium. Examination by microCT demonstrated retardation of decline volume Hounsfield unit values. An attendant reduction rate accumulation biodegradation pro...

Abstract In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with both detection techniques such as diode-array fluorescence (HPLC-DAD-FLD) for the determination of nesfatin-1 in fetal bovine serum samples. The limit (LOD) quantification (LOQ) were set at satisfactory values range 0.22–0.35 mg mL −1 0.67–1.05 , respectively (at two different ...

conclusions the prepared antibody can be used for the development of an elisa kit to assay afm1 in milk and other biological fluids. background aflatoxins are the most extensively studied group of mycotoxins produced by molds, especially the aspergillus group, which are highly toxic to animals and humans. objectives since immunoassay is a simple and rapid method for the analysis of many toxic s...

Human mesenchymal stem cells (MSCs) are capable of being used in human therapeutic procedures such as tissue repair and gene therapy due to their multipotency. However, the expansion of MSCs is limited by the restricted use of fetal bovine serum (FBS) as the nutritional supplement. Hence, contamination by pathogens from the xenogenic component can be eliminated if a serum-free media were used. ...

Serum contains iron-binding proteins, which inhibit the growth of most pathogenic microorganisms, including fungi. The purpose of this research was to investigate the effect of serum on growth of the opportunistic fungal pathogen Aspergillus fumigatus. Supplementing minimal essential medium (MEM) with up to 80% human serum or up to 80% fetal bovine serum (FBS) stimulated growth and increased th...