Serum contains iron-binding proteins, which inhibit the growth of most pathogenic microorganisms, including fungi. The purpose of this research was to investigate the effect of serum on growth of the opportunistic fungal pathogen Aspergillus fumigatus. Supplementing minimal essential medium (MEM) with up to 80% human serum or up to 80% fetal bovine serum (FBS) stimulated growth and increased th...

dietary flavonoids show beneficial effects in the prevention of chronic diseases. however, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. in the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (bsa) were investigated by fluorescence spectroscopy. the binding affinities of t...

MCF-7 human breast cancer cells, grown in long-term tissue culture, were found to be highly responsive to prolactin in terms of growth even in the presence of serum. Human prolactin, placenta!lactogen, and growth hormone (50-250 ng/ml) stimulated MCF-7 cells to grow when added to culture medium of cells in the presence of charcoal-stripped serum. Within 3 days of the hormone addition, a 4.4-fol...

Objective: To investigate the effect of small nucleolar host gene 12 (SNHG12) on migration and invasion human lung cancer cells by regulating angiostatin binding protein through microribonucleic acid (microRNA)-497. Methods: A549, H1299, PC9 were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium containing 10% fetal bovine serum, bronchial epithelial (HBE) Dulbecco’s modified eagle...

MCF-7 human breast cancer cells, grown in long-term tissue culture, were found to be highly responsive to prolactin in terms of growth even in the presence of serum. Human prolactin, placental lactogen, and growth hormone (50-250 ng/ml) stimulated MCF-7 cells to grow when added to culture medium of cells in the presence of charcoal-stripped serum. Within 3 days of the hormone addition, a 4.4-fo...

Background: Fibroblast cell lines have different application in biological research including nuclear transfer in cloning, feeder layer of embryonic stem cells, wound healing research and tissue engineering. The objective of the present study was to determine the proper pregnancy age for collecting and culturing guinea pig fetal fibroblast. Materials and Methods: Guinea pig fetuses were collect...

A cell line has been established from human placentae at the first trimester of normal pregnancy. The cell line was obtained by culture of purified cytotrophoblast cells in serum-free medium supplemented with epidermal growth factor, insulin, dexamethasone and 0.1% bovine serum albumin. The cells can be subcultured for >30 passages in one to three splits. All the cells were mononuclear epitheli...

Background: Protein extracts from chicken egg whites were prepared by utilizing different solvents and we studied the differences in proliferation. Methods: The white extract was using lysate, phosphate-buffered saline (PBS), pure water. vitro mixture experiment carried out to observe effect of preparations on proliferation cells. samples divided into following groups: control group with media,...