نتایج جستجو برای: fermentor
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Extracellular inulinase (E.C. 3.2.1.7) produced by Aspergillus niger 20 OSM culture in a 2-litre fermentor was isolated and purified by ion exchange chromatography on DEAE Sepharose, hydrophobic interaction on phenyl Sepharose, chromatofocusing on PBE-94, and size exclusion chromatography (SEC) on Sephadex G-200. The enzyme was homogeneous, as measured by SDS-PAGE with an apparent molecular mas...
Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris.
A gene (lsd1) encoding dextranase from Lipomyces starkeyi KSM22 has been previously cloned, sequenced, and expressed in Saccharomyces cerevisiae. The gene consisting of 1,824 base pairs and encoding a protein of 608 amino acids was then cloned into and secretively expressed in Pichia pastoris under the control of the AOX1 promoter. The dextranase productivity of the P. pastoris transformant (pP...
There are three variations of the fermentation process. In batch fermentation, a sterilized nutrient solution in the fermentor is inoculated with micro-organisms and incubation is allowed to proceed. During the course of the fermentation, oxygen is added (as in the case of aerobic microorganisms) and an acid or base to control the pH. The composition of the culture medium, the biomass concentra...
extract 0.3%, yeast extract 0.5% and CaCO30.4%, pH 7.0, incubated on a reciprocal shaker (300 rpm) at 27°C for 48hours. The culture broth was transferred into a 500 ml-Erlenmyer flask containing 100ml of the same seed medium, and incubated at 27°C for 48 hours on a rotary shaker (210rpm). Vegetative seed culture (2%, v/v) was transferred into a 30-liter jar fermentor containing 20 liters of a p...
13 Cellulase production in cultures of Acremonium cellulolyticus was significantly 14 improved by using waste milk pack (MP) that had been pretreated with cellulase. When 15 MP cellulose pretreated with cellulase (3 FPU/g MP) for 12 h was used as the sole 16 carbon source for A. cellulolyticus culture in a 3-L fermentor, the cellulase activity was 17 16 FPU/ml. This was 25-fold higher (0.67 FPU...
Sphaerobacter thermophilus Demharter et al. 1989 is the sole and type species of the genus Sphaerobacter, which is the type genus of the family Sphaerobacteraceae, the order Sphaerobacterales and the subclass Sphaerobacteridae. Phylogenetically, it belongs to the genomically little studied class of the Thermomicrobia in the bacterial phylum Chloroflexi. Here, the genome of strain S 6022(T) is d...
A simple method for the purification of staphylococcal enterotoxins A (SEA), B (SEB), and C2 (SEC2) from fermentor-grown cultures was developed. The toxins were purified by pseudo-affinity chromatography by using the triazine textile dye "Red A" and gave overall yields of 49% (SEA), 44% (SEB), and 53% (SEC2). The purified toxins were homogeneous when analyzed by sodium dodecyl sulfate-polyacryl...
Candida tropicalis can use phenol as the sole carbon and energy source. Experiments regarding phenol degradations from the water phase were carried out. The fermentor was operated as a fed-batch system with oxistat control. Under conditions of nutrient limitation and an excess of oxygen the respiration activity of cells was suppressed and some color metabolites (black-brown) started to be forme...
Fermentation systems are used to provide an optimal growth environment for many different types of cell cultures. The ability afforded by fermentors to carefully control temperature, pH, and dissolved oxygen concentrations in particular makes them essential to efficient large scale growth and expression of fermentation products. This video will briefly describe the advantages of the fermentor o...
Lipase production by Acinetobacter radioresistens was performed in a 2-L tank fermentor equipped with a nonwoven fabric, which was made of nylon 6 fiber and coated with a hydrophobic acrylic resin. The fermentation medium contained 2% (v/v) n-hexadecane as the carbon source. The use of the nonwoven fabric was intended for the dispersion of hydrocarbons; thus, the contact surface for the cells t...
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