The dorsal anterior region of the follicle cells (FCs) in the developing Drosophila egg gives rise to the respiratory eggshell appendages. These tubular structures display a wide range of qualitative and quantitative variations across Drosophila species, providing a remarkable example of a rapidly evolving morphology. In D. melanogaster, the bone morphogenetic protein (BMP) signaling pathway is...

In 1984, the first flow cytometry data file format was proposed as Flow Cytometry Standard 1.0 (FCS1.0). FCS 1.0 provided a uniform file format allowing data acquired on one computer to be correctly read and interpreted on other computers running a variety of operating systems. That standard was modified in 1990 and adopted by the Society of Analytical Cytology as FCS 2.0. Here, we report on an...

Fairy Circles (FCs) are barren circular patches of soil surrounded by grass species, the origin of which is poorly understood. FCs feature in both the gravel plains and dune fields of the Namib Desert. While a substantial number of hypotheses to explain the origin and/or sustainability of fairy circles have been presented, none are completely consistent with either their properties or distribut...

One of the major obstacles to obtaining human cells of a defined and reproducible standard suitable for use as medical therapies is the necessity for FCS (fetal calf serum) media augmentation in routine cell culture applications. FCS has become the supplement of choice for cell culture research, as it contains an array of proteins, growth factors and essential ions necessary for cellular viabil...

Fluorescence correlation spectroscopy (FCS) is an important spectroscopic technique which can be used for measuring the diffusion and thus size of fluorescing molecules at pico- to nanomolar concentrations. Recently, we introduced an extension of conventional FCS, which is called dual-focus FCS (2fFCS) and allows absolute diffusion measurements with high precision and repeatability. It was show...

Multi-photon (MPE) NIR excitation of fluorophores--attached as labels to biopolymers like proteins and nucleic acids, or bound at specific biomembrane sites-is one of the most attractive options in biological applications of laser scanning microscopy. Many of the serious problems encountered in spectroscopic measurements of living tissue, such as photodamage, light scattering and auto-fluoresce...

It is believed that the performance of Construction Management (CM) students in their undergraduate study is dependent on their mathematics and physics performance. A Fundamental of Construction Science (FCS) course, offered for freshman, covers materials related to mathematics and physics. CM students must perform better in mathematics and physics to perform better in FCS course. This study ai...

Fluorescence Correlation Spectroscopy (FCS) was first introduced by Elson, Madge and Webb (1-5) for studying the binding process between ethydium bromide and DNA. When the ethydium dye binds to DNA its fluorescence quantum yield changes by a large factor. It is essentially not fluorescent when free in solution and it becomes strongly fluorescent when bound to double strand DNA. Although the pro...

OBJECTIVE The purpose of this article was to determine the relationships among total body fat, visceral adipose tissue (VAT), fat cell size (FCS), ectopic fat deposition in liver (intrahepatic lipid [IHL]) and muscle (intramyocellular lipid [IMCL]), and insulin sensitivity index (S(i)) in healthy overweight, glucose-tolerant subjects and the effects of calorie restriction by diet alone or in co...

Poly (2-hydroxyethyl methacrylate) (HEMA) has been used as a clinical material, in the form of a soft hydrogel, for various surgical procedures, including endovascular surgery of liver. It is a clear liquid compound and, as a soft, flexible, water-absorbing material, has been used to make soft contact lenses from small, concave, spinning molds. Primary rat hepatocyte spheroids were created on a...