The effects of cAMP-analogue dibutyryl-cAMP and anticalmodulin W-7 were studied on de novo synthesis and secretion of lipids in cultures of hepatoma McArdle RH7777 cells and normal rat hepatocytes. Dibutyryl-cAMP and W -7 separately caused a significant decrease in the secretion of de novo synthesized triacyl [3H]glycerol in both cultures of McArdle cells and rat hepatocytes. The inhibito...

In a collaborative knowledge creation environment knowledge is socially constructed and the nature of knowledge being shared, integrated and converted is dominantly tacit. Achieving the required level of exchange, proliferation and extraction of tacit knowledge in a social network context is intimately tied to social capital and the socialization and externalization modes of knowledge conversio...

The lipid composition of the leaves of hardy Vernal and cold-sensitive Caliverde alfalfa plants, grown at different temperatures, was determined. Phosphatidyl glycerol, phosphatidyl inositol, and the sulfolipid content were directly related to growth temperature. Mono- and digalactose diglyceride and phosphatidyl choline and ethanolamine were inversely related to temperature. At corresponding g...

The profound neuroprotection observed in poly(ADP-ribose) polymerase-1 (PARP-1) null mice to ischemic and excitotoxic injury positions PARP-1 as a major mediator of neuronal cell death. We report here that apoptosis-inducing factor (AIF) mediates PARP-1-dependent glutamate excitotoxicity in a caspase-independent manner after translocation from the mitochondria to the nucleus. In primary murine ...

BACKGROUND It is believed that the oxidatively modified lipoproteins play a critical role in activating platelets and is a contributing factor in the etiology of a number of cardiovascular-related diseases. OBJECTIVE Identify the active component(s) of oxidized LDL that initiated shape change in plasma-free human platelets prepared by a gel filtration method Material and Method: Shape change ...

mTOR, the mammalian target of rapamycin, is a conserved serine/threonine kinase which belongs to the phosphatidyl-linositol kinase-related kinase (PIKK) family. It has two complexes called mTORC1 and mTORC2. It is well established that mTOR plays important roles in cell growth, proliferation and differentiation. Over-activation of the mTOR pathway is considered to have a relationship with the d...

The synthesis of phosphatidylserine in two gram-positive aerobic bacteria has been partially characterized. We have located a cytidine 5'-diphospho-diacylglycerol:L-serine O-phosphatidyltransferase (phosphatidylserine synthase) activity in the membrane fraction of Bacillus licheniformis and Bacillus subtilis. The activity was demonstrated to be membrane associated by differential centrifugation...

Adverse outcome of pregnancy is a potentially dangerous complication of conception and affects 15-20% of pregnancies. According to recent studies increased level of blood antiphospholipids antibodies, if accompanied by predisposing factors, can be an adverse event in human pregnancy. The aim of the present study was to compare the maternal serum levels of anticardiolipin and antiphosphatidyl se...