brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

cloning and expression of the l-phenylalanine dehydrogenase gene, from b. sphaericus in e. coli were done. the gene was cloned in the vector pet16b and transformed into e. coli bl21 (de3). the functional form of the l-phenylalanine dehydrogenase enzyme was purified by affinity purification techniques, taking advantage of the ability of this enzyme to bind to the nucleotide site affinity dye, re...

background and objectives: clostridium perfringens, a gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. c. perfringens is subdivided into five types a to e based on its four major alpha, beta, epsilon and iota toxins. the aim of the present study was cloning and expression of c. perfringens type d vaccine strain epsilon...

objective(s): streptokinase (ska) is an antigenic protein which is secreted by streptococcus pyogenes. streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. in the present study, recombinant streptokinase from s. pyogenes was produced and showed that recombinant ska protein was recognized by infected human sera using western blot analysi...

embryonic stem cells (escs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. also, these cells have the self-renewing ability. escs could differentiate into most of cell lineages. lymphocytes generated from hematopoietic stem cells. we designed a feeder free culture condition to generate lymphoid lineage cells from escs. we demonstrated that il-7 plays cr...

ganoderma lucidum is one of the most well-known fungi, and has many applications in medicine. ganoderic acid is among the valuable secondary metabolites of ganoderma lucidum, and responsible for the inhibition of the tumor cell growth and cancer treatment. application of ganoderic acid has been limited because of low yields of its production from ganoderma lucidum. the present study aims to inv...

background: the effect of curcumin as a natural safe compound with different biological activities was examined on fungal growth and aflatoxin production in aspergillus parasiticus nrrl 2999. methods: the fungus was cultured in presence of serial two-fold concentrations of curcumin (125-2000 µg/ml) in yeast extract sucrose broth for 3 days at 28°c. mycelia dry weight was determined as an index ...

dna microarray is a powerful approach to study simultaneously, the expression of 1000 of genes in a single experiment. the average value of the fluorescent intensity could be calculated in a microarray experiment. the calculated intensity values are very close in amount to the levels of expression of a particular gene. however, determining the appropriate position of every spot in microarray im...

background bovine viral diarrhea (bvd) is an economically important disease of cattle with a worldwide distribution. diagnosis of bvd relies on laboratory-based detections of its viral causing agent or virus specific antibodies. the most common laboratory method used for this purpose is the elisa. bovine viral diarrhea virus (bvdv) nonstructural protein 3 (ns3) is one of the most highly conserv...

conclusions although the data support the idea that the basic mechanism of imipenem resistance could be via the loss of oprd, they do not fully explain the role of oprd and indicate that other mechanisms may play an important role. additionally, the significant decrease in the oprd levels in mdr strains suggests that oprd also plays a role in the emergence of both carbapenem and non-carbapenem ...