Through the flow injection analysis experiments, we discovered that an unsaturated complex of Cu(II) and polyamines (spermine, spermidine, putrescine) had a strongly catalytic effect on luminol-H(2)O(2) chemiluminescence (CL) reaction, and that the CL intensity is proportional to the concentrations of polyamines. Based on the automatic formation of an unsaturated complex of polyamines and Cu(II...

The natural polyamines spermine and spermidine, the biosynthetic precursor putrescine and their analogues cadaverine and tyramine stimulate the GTPase activity of purified GTP-binding proteins (Go/Gi) from calf brain reconstituted into phospholipid vesicles. The order of potency was spermine greater than spermidine greater than putrescine = cadaverine greater than tyramine. The physiological re...

Pea is a seed legume. It rich in cellulose fibre and protein. also significant source of minerals vitamins. In this paper, we set out to better characterize the physiological responses Pisum sativum L. combined effects NaCl, 100 mM gibberellins (GA3). Our analysis revealed that NaCl caused decrease growth resulting reduction root elongation, distribution density, leaf number area, dry matter ro...

The effects of polyamines (putrescine, spermidine and spermine) on glutathione reductase (glutathione: NADP+ oxidoreductase, EC 1.8.1.7; GR) activity of spinach leaves (Spinacia oleracea L. cv. Gladiator) were investigated under in vivo and in vitro conditions. Spinach was grown in sand culture under controlled conditions for 30 d. In in vivo assays 30-day-old plants were sprayed with polyamine...

We have examined whether dietary polyamines influence the formation and initial growth of azoxymethane (AOM)-induced aberrant crypt foci (ACF) in rat colon. Effects of a combination of dietary polyamines at three dose levels (putrescine: 50, 280, 740 nmol/g; spermidine: 10, 261, 763 nmol/g; spermine: 1, 31, 91 nmol/g) in the polyamine-poor AIN-76A diet were studied in animals in two different e...

After feeding of rats with the raw soyabean diet, serum polyamine concentrations (primarily spermidine) were significantly increased at 6 and 12 h (Table I ) . N o similar increases were apparent in the control animals. After 24 h, the circulating levels of polyamines returned to control values and subsequently the total polyamine content in the blood was below normal. These later changes sugge...

The polyamines spermine and spermidine and the diamine putrescine inhibit lipid peroxidation in phospholipid liposome suspensions and rat liver homogenates. Using the chemiluminescence technique the antioxidant activity of polyamines was found to be due to reactions with the free radical intermediates of lipid peroxidation and/or superoxide radicals. Also, the antioxidant action of polyamines c...

Polyamines have been associated with a large number of plant growth and developmental processes. In particular, they have been associated with floral initiation with increased polyamines concentration occurring during flowering in horticulture plants. However, there is limited information about polyamines in cotton (Gossypium hirsutum L.) and how they are distributed in the cotton plant. In add...

Polyamines are essential compounds for cell survival and have key roles in plant stress protection. Current evidence points to the occurrence of intricate cross-talks between polyamines, stress hormones and other metabolic pathways required for their function. In this review we integrate the polyamine metabolic pathway in the context of its immediate metabolic network which is required to under...

Background. Indole and polyamines are involved in the regulation of physiological processes bacteria associated with adaptation to stress, biofilm formation, antibiotic tolerance, bacterial persistence. However, molecular targets mechanisms action these metabolites still poorly understood. In this work, we studied effect indole on expression such genes as: rpoS, relA, spoT, encoding regulators ...