Hepatocyte gap junction proteins, connexins (Cxs) 26 and 32, are down regulated during obstructive cholestasis (OC) and lipopolysaccharide hepatocellular cholestasis (LPS-HC). We investigated rat hepatic Cxs during ethynylestradiol hepatocellular cholestasis (EE-HC) and choledochocaval fistula (CCF) and compared them with OC and LPS-HC. Levels (immunoblotting) and cellular distribution (immunof...

Steroid estrogens (SEs) are mainly derived from livestock manure, and composting is the common method of bioresource utilization. In this study, an open-air experiment with dairy manure was conducted to observe degradation loss five SEs under influence different precipitation intensities additional mixed fermentation strains. SE determination results for after 30 days indicated that average rem...

Hormones 17α-ethinylestradiol, 17β-estradiol and estriol were analyzed in urban water supply due to the concern about the problem associated with the presence of these compounds in water bodies. The aims of this project were adapt, validate analytical methodology and monitor these hormones in drinking water. Results showed that the limits of detection, linearity, regression coefficients and rec...

This study was to investigate the effect of annexin A5 on testosterone secretion from primary rat Leydig cells and the underlying mechanisms. Isolated rat Leydig cells were treated with annexin A5. Testosterone production was detected by chemiluminescence assay. The protein and mRNA of Steroidogenic acute regulatory (StAR), P450scc, 3β-hydroxysteroid dehydrogenase (3β-HSD), 17β-hydroxysteroid d...

17α-ethinylestradiol (EE2) is a recalcitrant micropollutant which is found in small amounts in municipal wastewater. But these small amounts still adversely affect for the reproductive function of aquatic organisms. Evidence in the past suggested that full-scale WWTPs equipped with nitrification process enhanced the removal of EE2 in the municipal wastewater. EE2 has been proven to be able to b...

It is now established that the brain and other nervous systems have the capability of forming steroids de novo, the so-called "neurosteroids." The pioneering discovery of Baulieu and his colleagues, using rodents, has opened the door to a new research field of "neurosteroids." In contrast to mammalian vertebrates, little has been known regarding de novo neurosteroidogenesis in the brain of bird...

In European aquaculture, Eurasian perch, Perca fluviatilis L., is perceived as one of the most highly valuable freshwater fish species and a strong candidate for development aquaculture. pursuit improving quality reproduction in this domesticated species, investigating hormones mediating final oocyte maturation (FOM) therefore indispensable. But, exact nature maturation-inducing hormone (MIH) p...