نتایج جستجو برای: ethidium bromide

تعداد نتایج: 17729  

Journal: :Nucleic acids research 1982
S A Winkle L S Rosenberg T R Krugh

The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms ...

2009
A. I. Dragan R. J. Strouse C. D. Geddes

Ethidium bromide (EB) is a commonly used probe for fluorescence detection and quantification of nucleic acids, since EB forms a highly luminescent complex with dsDNA. Typical detection sensitivity of EB-based assays to dsDNA in solution is about 0.1 lg. With the aim to increase the sensitivity of EB assays we have employed the Metal-Enhanced Fluorescence technology. We show that deposition of t...

2010
Arnab Roy Chowdhury Rahul Bakshi Jianyang Wang Gokben Yildirir Beiyu Liu Valeria Pappas-Brown Gökhan Tolun Jack D. Griffith Theresa A. Shapiro Robert E. Jensen Paul T. Englund

Introduced in the 1950s, ethidium bromide (EB) is still used as an anti-trypanosomal drug for African cattle although its mechanism of killing has been unclear and controversial. EB has long been known to cause loss of the mitochondrial genome, named kinetoplast DNA (kDNA), a giant network of interlocked minicircles and maxicircles. However, the existence of viable parasites lacking kDNA (dyski...

Journal: Journal of Nuts 2012
A. Mousavi B. Mohammadparast M. Rasouli P. Martínez-Gómez

Simple sequence repeat (SSR markers or microsatellites), based on the specific PCR amplification of DNA sequences, are becoming the markers of choice for molecular characterization of a wide range of plants because of their high polymorphism, abundance, and codominant inheritance. Different methods have been used for the analysis of the SSR amplified fragments being submarine agarose electropho...

Journal: :BioTechniques 1996
O Enger

Exact quantification of the amount of amplified polymerase chain reaction (PCR) product is a crucial element in quantitative PCR approaches. Normally such quantification is based on the application of radioactive(2) or fluorochrome-labeled primers (4), HPLC (3) or densitometric-scanning of an agarose gel after staining with dyes like ethidium bromide (1). These quantification techniques often r...

1998
Samir Kumar Pal Debabrata Mandal Kankan Bhattacharyya

Photophysical processes of ethidium bromide (EB) in homogeneous solutions, micelles, and reverse micelles have been investigated. In acetonitrile, the fluorescence intensity and lifetime of EB are 6.3 ( 0.3 times those in water and 1.25 ( 0.1 times those in acetone. This is attributed to the weaker hydrogen-bond acceptor property of acetonitrile, compared to water and acetone. Addition of water...

Journal: :Journal of Biological Engineering 2009
Laura Paixão Liliana Rodrigues Isabel Couto Marta Martins Pedro Fernandes Carla CCR de Carvalho Gabriel A Monteiro Filipe Sansonetty Leonard Amaral Miguel Viveiros

BACKGROUND Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy. The development of methods for the early detection and quantification of drug transport across the bacterial cell wall is a tool essential to understand and overcome this type of drug resistance mechanism. This approach was developed to s...

2013
Jianwei Zhou Cui Kong Bo Ban Lin Sun Cuiyun Dou Zhaocai Zhang

Objective: To explore a fast and sensitive method for screening T cell receptor (TCR) biases through comparison of silver staining for polyacrylamide gel electrophoresis and ethidium bromide staining for agarose gel electrophoresis. Methods: The lymphocytes were isolated from peripheral blood which collected from ten patients with T1DM; the total RNA was extracted followed by transformed to cDN...

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