The aim of this research was to enhance bioethanol production via immobilization and microencapsulation of Saccharomyces cerevisiae CICC 1001 into delignified cellulosic material (DCM), prepared from sawdust and Na alginate. It has been demonstrated that microencapsulation process using Alginate: DCM ratio of (1:1.5) and alginate solution of 2.5% by wt, attained the highest microencapsulation e...

BACKGROUND Switchgrass is an abundant and dedicated bioenergy feedstock, however its inherent recalcitrance is one of the economic hurdles for producing biofuels. The downregulation of the caffeic acid O-methyl transferase (COMT) gene in the lignin pathway of switchgrass reduced lignin content and S/G ratio, and the transgenic lines showed improved fermentation yield with Saccharomyces cerevisi...

Water hyacinth biomass is a potential substrate for the production of ethanol which can be conveniently used as a gasoline additive as an octane booster and improve the vehicle emissions. The present study is focused on isolating and screening indigenous fungi for the hydrolysis of water hyacinth in the lignocellulosic conversion process. The initial cellulose activity was measured as ICMC (car...

Cofactor imbalance impedes xylose assimilation in Saccharomyces cerevisiae that has been metabolically engineered for xylose utilization. To improve cofactor use, we modified ammonia assimilation in recombinant S. cerevisiae by deleting GDH1, which encodes an NADPH-dependent glutamate dehydrogenase, and by overexpressing either GDH2, which encodes an NADH-dependent glutamate dehydrogenase, or G...

In this work, Saccharomyces cerevisiae strains PE-2 and CAT-1, commonly used in the Brazilian fuel ethanol industry, were engineered for xylose fermentation, where the first fermented xylose faster than the latter, but also produced considerable amounts of xylitol. An engineered PE-2 strain (MEC1121) efficiently consumed xylose in presence of inhibitors both in synthetic and corn-cob hydrolysat...

Bio-ethanol production from lignocellulosic raw materials could serve as a sustainable potential for improving the supply of liquid fuels in face of the food-to-fuel competition and the growing energy demand. Xylose is the second abundant sugar of lignocelluloses hydrolysates, but its commercial-scale conversion to ethanol by fermentation is challenged by incomplete and inefficient utilization ...

Abstract Background Ethanol biorefineries need to lower their overall production costs become economically feasible. Two strategies achieve this are reduce using cheaper feedstocks or increase the ethanol yield. Low-cost usually have high non-structural components (NSC) content; therefore, a new process is necessary accommodate these and overcome negative effects of NSC. This study developed no...

Propanol appeared better than ethanol for the long-term storage of spinach leaves, followed by hexanol and pentanol. The lowest molecular weight and yields of DNA came from spinach stored in methanol and ethanol. In an experiment with spinach leaves stored in 100, 95, 70, 50 and 25% ethanol, the 50% ethanol stored leaves appeared to yield more and higher molecular weight DNA than any other trea...

The hydrolysis of inulin from Jerusalem artichoke (JA) slices by HCl under different regimes of temperature and hold (contact time) time was investigated. Final reducing sugars concentration in the hydrolyzates depended on temperature, pH, hydromodule (JA:water) and hold time. Acid hydrolysis at higher temperature and longer hold time increased the degradation of fructan to fructose and increas...