OBJECTIVE To determine if a new inhibitor, esculetin (EST), can block resorption of cartilage. METHODS Interleukin 1alpha (IL1alpha, 0.04-5 ng/ml) and oncostatin M (OSM, 0.4-50 ng/ml) were used to stimulate the release of proteoglycan and collagen from bovine nasal cartilage and human articular cartilage in explant culture. Proteoglycan and collagen loss were assessed by dimethylmethylene blu...

Endoscopic sphincterotomy (EST) and endoscopic papillary balloon dilatation (EPBD) have become the main therapeutic procedures in the treatment of biliary and pancreas disease. The risk of cholangiocarcinoma (CCA) is not well investigated among post-EST/EPBD patients with benign diseases, particularly in Asia population. A retrospective nationwide cohort study using data from Taiwan's National ...

The archaeological research from 2016-2021 allowed us to identify in Buridava some types of structures that can also be found other sites belonging the classic Geto-Dacian era and which largely correspond discoveries Dumitru Berciu 1961-1992. were made on terrace VIII, last one western side bushy hills Cosota. Looking back benefiting today multiple sources analysis (LIDAR, aerial photography, e...

OBJECTIVE To evaluate the safety and efficacy between endoscopic papillary balloon dilatation (EPBD) and endoscopic sphincteropapillotomy ( EST) for common bile duct stones using meta-analysis method. METHODS Randomizd controlled trials comparing EPBD with EST for common bile duct stones and published from January 1990 to July 2012 were recruited. This meta-analysis was conducted to estimate ...

The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those marke...

While genome sequencing projects are advancing rapidly, EST sequencing and analysis remains a primary research tool for the identification and categorization of gene sequences in a wide variety of species and an important resource for annotation of genomic sequence. The TIGR Gene Indices (http://www.tigr.org/tdb/tgi. shtml) are a collection of species-specific databases that use a highly refine...

Zymogram analysis using isoelectric focusing on polyacrylamide gels was performed to characterize and distinguish two Toxoplasma gondii isolates ("strains" BK and RH). The activity of the following 14 enzymes in the cell lysates was investigated: IDH, MDH, ME, 6PG, G6P, LDH, IPO, HEX, PGM, EST, ALP, ACP, LAP, and PGI. Nine enzymes (IDH, G6P, LDH, HEX, PGM, EST, ALP, ACP, and PGI) showed distinc...

introduction - les impuretes, meme en faibles teneurs, modifient beaucoup les proprietes physiques des metaux. ainsi, pour une etude profonde de ces proprie, tes , on a besion d'un metal extremement pur. la methode de la zone fondue a permis cette realisation . principe - dans la methode de purification dite de la «zone fondue» decouverte par pfann en 1952, une courte zone liquide est depl...