In this study, Limosilactobacillus fermentum PTCC 1638 and Lacticaseibacillus rhamnosus 1637 were used alone in combination to ferment quinoa seeds, the effect of fermentation (37 °C; 24 h) on pH, total phenols, tocopherols, vitamin C, antioxidant activity, enzymes inhibition (α-amylase α-glucosidase; antidiabetic effect) was investigated. The results showed that with increase time, bacterial p...

The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibrat...

The DNA-binding mode of archaeal feast/famine-regulatory proteins (FFRPs), i.e. paralogs of the Esherichia coli leucine-responsive regulatory protein (Lrp), was studied. Using the method of systematic evolution of ligands by exponential enrichment (SELEX), optimal DNA duplexes for interacting with TvFL3, FL10, FL11 and Ss-LrpB were identified as TACGA[AAT/ATT]TCGTA, GTTCGA[AAT/ATT]TCGAAC, CCGAA...

An experiment was conducted in the Biological Dep. College of Science, Kufa University to determine the antagonism activity of Lemon and Lime juices against some bacteria e.g. Esherichia coli, Klebsiella pneumonia, and Shigella flexneri. Results of this experiment revealed that all juices concentrations have inhibiting effects against bacteria due to the presence of citric acid and some volatil...

Amphibian skin secretions contain biologically-active compounds, such as anti-microbial peptides and trypsin inhibitors, which are used by biomedical researchers as a source of potential novel drug leads or pharmacological agents. Here, we report the application of a recently developed technique within our laboratory to "shotgun" clone the cDNAs encoding two novel but structurally-related pepti...