Nowadays the extensive use of antibiotics for the treatment of humans and warm blooded animals has influenced the frequency and spread of antibiotic resistant E. coli O157:H7 in different aqueous sources. Thus, the main aim of this study was to evaluate the antibiotic susceptibilities of E. coli O157:H7 isolated from different water sources and establish a correlation between the presence of vi...

background:urinary tract infections (utis) are the most common infectious diseases in childhood. the uropathogenic escherichia coli (upec) strains account for as much as 80% of utis. objective:from a clinical perspective, it is important to know which virulence factors and antibiotic resistance properties are present in upec strains in pediatrics. therefore, this study was carried out to invest...

Urinary tract infections primarily caused by uropathogenic strains of Escherichia coli (E. coli) remain a significant public health problem in both developed and developing countries. An important virulence determinant in uropathogenesis is biofilm formation which requires expression of fimbriae, flagella, and other surface components such as lipopolysaccharides. In this study, we explored the ...

The enterotoxigenic and porcine enteropathogenic Escherichia coli (EtEc and PEPEc) strains are agents associated with swine neonatal diarrhea, causing economic losses in swine production. The main goal of this study was to identify virulence genes of EtEc, verotoxigenic (VtEc) and PEPEc in intestinal strains responsible for swine diseases, by molecular typing using Pcr in newborn piglets from a...

A multiplex PCR assay was developed which allowed the simultaneous detection of five trait genes or virulence markers in enterohemorrhagic Escherichia coli (EHEC) serotypes. A primer pair, designed to detect a single base-pair mutation in the uidA gene, is specific only for the prototypic EHEC of O157:H7 serotype and its toxigenic, non-motile variants. In a similar way, primers to the eaeA gene...

BACKGROUND Pathogenic strains in Escherichia coli can be divided into several pathotypes according to their virulence features. Among them, uropathogenic E. coli causes most of the urinary tract infections and has a genotype distinct from other virulent strains of E. coli. In this study, we sequenced and analyzed the genome of E. coli NCCP15653 isolated from the feces of a diarrhea patient in 2...

The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in Escherichia coli isolated from canine diarrheal cases. Fecal samples were collected 77 dogs with clinical diarrhea 10 non-diarrheic dogs. Breed, age sex, manifestations recorded. Samples plated on MacConkey EMB agars, followed by standard isolation procedures identification E. coli. An ...

This study aimed to evaluate the most predominant strain of avian pathogenic Escherichia coli (APEC) that causes colibacillosis with respiratory manifestation in broiler chickens. Isolation and serotyping identification for isolates E. were performed. Prevalence APEC differentiated by detection 8 virulence-associated genes “astA, iss, irp2, papC iucD, tsh, vat, cva/cvi” using multiplex PCR tech...

Background and Aim: The pathogenicity of Escherichia coli is determined by the presence genes that mediate virulence factors such as adherence capacity toxin production. This research aimed to identify adhesion antibiotic resistance E. strains associated with diarrhea in piglets Colombia. Materials Methods: Presumptive were isolated from rectal swabs swine farms between 4 40 days age evidence d...

This study was conducted to characterize virulence genes of Escherichia coli isolates from water, sediment, fish, and crab in Aby Lagoon. Serogrouping was performed by EPEC antisera in 113 E. coli strains. The presence of diarrhea-associated genes (eae, stx, AggR, elt, and est) was assessed by multiplex PCR using specific primers. Based on the multiplex PCR, sixty-two isolates (42 from water, 1...