Voriconazole (UK-109,496) is a novel triazole derivative with potent broad-spectrum activity against various fungi, including some that are inherently resistant to fluconazole, such as Candida krusei. In this study we compared the effect of subinhibitory concentrations of voriconazole and fluconazole on sterol biosynthesis of fluconazole-resistant and -susceptible Candida albicans strains, as w...

The title ergosterol compound, C(28)H(44)O(4)·H(2)O, is composed of four fused rings (three six-membered and one five-membered) and a side chain. It is a derivative of ergosterol and was isolated from a marine-derived halotolerant fungus, Cladosporium cladosporioides PXP-49. In the crystal, mol-ecules are assembled by classical O-H⋯O hydrogen bonds, forming a two-dimensional network, with base ...

Ketoconazole, an orally active antimycotic drug, is a potent inhibitor of ergosterol biosynthesis in Candida albicans when added to culture media which support yeast or mycelial growth or to cultures containing outgrown mycelium. This inhibition coincides with accumulation of sterols with a methyl group at C-14 and can thus be attributed to an interference with one of the reactions involved in ...

The isolation of steroid compounds from Adam Hawa leaves has been carried out through maceration, partition, separation, and purification. relatively pure BF2 isolate was 7 mg amorphous with a melting point 158-1700C. Results 1H-NMR spectrum analysis CDCl3 solvent, 500 MHz δH: 7,53 (d, J= 5,4 Hz, = C-H ); 7,72 5,7 C-H); 5,35 (1H, d, J=5,15 =C-H); 3,51 m, Ar-CH), 0,93 6,2 3H), 0,86 (3H, s), 0,84...

The effect of ergosterol depletion by ketoconazole on the leishmanicidal activity of the pore-forming antibiotic amphotericin B (AmB) was investigated. Leishmania mexicana promastigotes were lysed within minutes by the addition of micromolar concentrations of AmB (0.5 microM) but became insensitive to AmB after growth in the presence of ketoconazole (0.25 microM, 90 h). Lipid chromatographic an...

Inhibition of Δ(24)-sterol methyltransferase (24-SMT) in Sporothrix schenckii sensu stricto and Sporothrix brasiliensis was investigated in vitro. The effects on fungal growth and sterol composition of the 24-SMT inhibitor 22-hydrazone-imidazolin-2-yl-chol-5-ene-3β-ol (H3) were compared to those of itraconazole. MIC and MFC analysis showed that H3 was more effective than itraconazole against bo...

THE absorption spectrum of cod-liver oil possesses a quite special interest because of the fact that the oil contains three known important substances which are sensitive towards light, namely, vitamin A, ergosterol or provitamin D and vitamin D itself. A number of workers have studied the absorption spectrum of the medicinal oil, and Schlutz and his colleagues [1925, 1926] and Heilbron, Kamm a...

Methionine was found to be more efficient than formate, serine, bicarbonate, formaldehyde, or choline as a source of the C-28 methyl group of ergosterol in yeast (1). While transfer of an intact methyl group to carbon has not been previously recorded, oxidation of the methyl group of methionine to formate has been demonstrated and formate-Cl* has been shown to give rise to C-28 of ergosterol (2...