BACKGROUND To investigate if microRNAs (miRNAs) play a role in regulating h-ERG trafficking in the setting of chronic oxidative stress as a common deleterious factor for many cardiac disorders. METHODS We treated neonatal rat ventricular myocytes and HEK293 cells with stable expression of h-ERG with H2O2 for 12 h and 48 h. Expression of miR-17-5p seed miRNAs was quantified by real-time RT-PCR...

Solar magnetic tornadoes are known to be one of the mass and energy transport mechanisms from the lower solar atmosphere into the upper layers of the solar corona. A bright spiral structure with two arms is observed using high-cadence EUV images of 171, 193 and 304 Ǻ channels of Atmospheric Imaging Assembly (AIA) aboard the Solar Dynamics Observatory (SDO) on 10th of July 2011 for three hours. ...

Whether vitamin D is chemopreventive and/or has potential therapeutically in prostate cancer is unresolved. One confounding factor is that many prostate cancers express a TMPRSS2:ERG fusion gene whose expression is increased both by androgens and by vitamin D receptor (VDR) activation. Two challenges that limit VDR agonist use clinically are hypercalcemia and the cooperation of VDR with ERG to ...

The interaction of transcription factors with specific DNA sequences is critical for activation of gene expression programs. In endothelial cells (EC), the transcription factor NF-κB is important in the switch from quiescence to activation, and is tightly controlled to avoid excessive inflammation and organ damage. Here we describe a novel mechanism that controls the activation of NF-κB in EC. ...

half of all prostate cancers in the Western countries harbor gene fusions that involve regulatory sequences of the androgen receptor (AR)-responsive genes (predominantly TMPRSS2) and protein coding sequences of nuclear transcription factors of the ETS gene family (predominantly ERG). This leads to unscheduled androgen-dependent expression ofETS-related transcription factors in tumor cell-specif...

Electroretinography (ERG) is a well-established diagnostic procedure for objectively evaluating retinal function. In this study, ERG in beagle dogs, which are a popular experimental animal, was performed to determine the normal range of ERG variables and assess differences between the left and right eyes. ERG findings including rod, combined rod-cone, single-flash cone, and 30-Hz flicker respon...

Purpose: To assess differentially methylated "landscapes" according to prostate cancer Gleason score (GS) andERGoncogene expression status, and to determine the extent of polycomb group (PcG) target gene involvement, we sought to assess the genome-wideDNAmethylation profile of prostate cancer according to Gleason score and ERG expression. Experimental Design:Genomic DNA from 39 prostate cancer ...

The oncogenic TLS-ERG fusion protein is found in human myeloid leukemia and Ewing's sarcoma as a result of specific chromosomal translocation. To unveil the potential mechanism(s) underlying cellular transformation, we have investigated the effects of TLS-ERG on both gene transcription and RNA splicing. Here we show that the TLS protein forms complexes with RNA polymerase II (Pol II) and the se...

ERG (ETS-related gene) belongs to the ETS family of transcription factors, and has been recently reported to contribute to homeostatic balance in skeleton cell plasticity. MicroRNA-30 (miR-30) family is also demonstrated to play a role in controlling chondrocyte differentiation. The current study investigated the miR-30b and ERG expression in articular cartilage of osteoarthritis (OA) patients....

The morphology of the electroretinogram (ERG) can be altered as a result of normal and pathological processes of the retina. However, given that the ERG is almost solely assessed in terms of its amplitude and timing, defining the shape of the ERG waveform so that subtle, physiologically driven, morphological changes can be systematically and reproducibly detected remains a challenging problem. ...