نتایج جستجو برای: enzyme linked immunospot assay

تعداد نتایج: 620627  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2001
R Wang J Epstein F M Baraceros E J Gorak Y Charoenvit D J Carucci R C Hedstrom N Rahardjo T Gay P Hobart R Stout T R Jones T L Richie S E Parker D L Doolan J Norman S L Hoffman

We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-gamma responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8(+) ...

Journal: :hepatitis monthly 0
behrouz naeimi department of microbiology and parasitology, faculty of medicine, bushehr university of medical sciences, bushehr, ir iran; the persian gulf tropical medicine research center, bushehr university of medical sciences, bushehr, ir iran farnaz mazloom kalimani student research committee, bushehr university of medical sciences, bushehr, ir iran ali akbar pourfatolah department of immunology, faculty of medical sciences, tarbiat modares university, tehran, ir iran masoud azimzadeh student research committee, bushehr university of medical sciences, bushehr, ir iran alireza mankhian bushehr blood transfusion organization, bushehr, ir iran samad akbarzadeh department of biochemistry, faculty of medicine, bushehr university of medical sciences, bushehr, ir iran

conclusions high hev seroprevalence (16.7%) was observed among the blood donors in bushehr province. it appears that exposure to hev increases with age; although, more people should be examined. results overall, 105 (16.7%) blood samples were positive for hev-specific-igg antibodies, while 523 (83.8%) were negative. the presence of anti-hev igg was not associated with gender; however, it was co...

Journal: :jundishapur journal of microbiology 0
ramezan ali ataee department of medical microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran reyhane kashefi department of microbial biotechnology, faculty of science, payame noor university, tehran, ir iran; department of microbial biotechnology, faculty of science, payame noor university, tehran, ir iran. tel: +98-989122190418, fax: +98-2126127258 gholam hossein alishiri department of rheumatology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran davoud esmaieli department of medical microbiology, faculty of medicine, baqiyatallah university of medical sciences, tehran, ir iran

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease. the staphylococcal superantigens are considered as the causative agent of ra disease. objectives this study aimed to assess the presence of staphylococcal enterotoxin d in synovial fluid and blood of patients with ra. patients and methods a total of 120 blood and sf samples of patients with ra were studied. ba...

Journal: :archives of pediatric infectious diseases 0
shahnaz armin pediatric infections research center, shahid beheshti university of medical sciences, tehran, ir iran; pediatric infections research center, mofid children’s hospital, tehran, ir iran. tel: +98-9121099758, fax: +98-2122226941سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences) farhad mahvelati shamsabadi pediatric infections research center, shahid beheshti university of medical sciences, tehran, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences) azadeh kiomarci pediatric infections research center, shahid beheshti university of medical sciences, tehran, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences) farzane jadali pediatric infections research center, shahid beheshti university of medical sciences, tehran, ir iranسازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (shahid beheshti university of medical sciences)

background the etiology of the guillain-barre syndrome (gbs) is not clear; in many cases, gbs is triggered by a bacterial or viral illness. objectives to evaluate the role of active infection with herpesviridae group and its association with this syndrome we have investigated the cerebrospinal fluid (csf) and serum of patients with gbs for detection of these viruses. patients and methods in a pr...

Journal: :medical journal of islamic republic of iran 0
ghasem mosayyebi from the dept. of immunology, school of public health, tehran university of medical sciences jafar forghanizadeh the dept. of rheumatology, hazrate rasool hospital, iran university of medical sciences banafsheh gholestan the dept. of statistics and epidemiology, school of public health, tehran university of medical sciences, tehran, i.r. iran. fazel shokri from the dept. of immunology, school of public health, tehran university of medical sciences

total igm, iga, and igm-rheumatoid factor (rf) and iga-rf were quantitated in the serum and synovial fluid of 35 seropositive and 8 seronegative rheumatoid arthritis (ra) patients, using a sensitive enzyme-linked immunosorbent assay (elisa). mononuclear cells isolated from peripheral blood and synovial fluid of three seropositive patients were also stimulated in vitro with phorbol myristate ace...

Journal: :BioTechniques 1997
Y Cui L J Chang

Originally developed for detecting antibody production from B lymphocytes, the enzyme-linked immunospot (ELISPOT) assay was later modified to assess cytokine production from various immune effector cells. Although the ELISPOT assay can detect antibody or cytokine production at the single-cell level, the visual counting of spots in a 96-well plate under a microscope makes this method unsuitable ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس - دانشکده کشاورزی 1388

ویروس پیچیدگی زرد برگ گوجه فرنگی (tomato yellow leaf curl virus, tylcv)از جمله بیمارگرهای مخرب گوجه فرنگی است و باعث خسارت اقتصادی در سراسر جهان می شود. به دلیل تنوع و گستردگی این بیمارگر در ایران ردیابی ویروس برای کنترل آن ضروری است. از آنجائیکه روش الایزا (elisa) enzyme-linked immunosorbent assay روشی مناسب برای تشخیص ویروسها در سطوح وسیع است آماده سازی آنتی ژن مورد نیاز برای فرآیند تولید آنت...

Journal: :journal of reproduction and infertility 0

background: nowadays, chlamydia trachomatis is known as a causative agent of infertility. because of, asymptomatic nature of infection, many may suffer from its lasting complications such as infertility. this study was performed in tehran during april 2007 to april 2008 to compare the prevalence of chlamydia trachomatis infection in fertile and infertile women using elisa and pcr methods. metho...

Journal: :Journal of Investigative Dermatology 2013

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