We report here the results of the diagnostic performances of Vitros Syphilis TPA (a chemiluminescence treponemal assay) compared with those of two treponemal enzyme immunoassays and of traditional versus reverse syphilis algorithms. Ease of use, automation, and high throughput make the Vitros Syphilis TPA assay a good choice for syphilis screening in high-volume laboratories.

10 11 12 Daniel M. Musher,* Atisha Manhas, Pranav Jain, Franziska Nuila, 13 14 Amna Waqar, Nancy Logan, Bernard Marino, Edward A. Graviss, 15 16 17 From the Medical Service (Infectious Disease Section), Michael E. DeBakey 18 Veterans Affairs Medical Center, Houston, Texas, the Departments of Medicine 19 and Molecular Virology and Microbiology, Baylor College of Medicine, and the 20 Department o...

We studied the EMIT (Enzyme Multiplied Immunoassay Technique, Syva) procedures for the assay of phenytoin and phenobarbital in serum, adapting them to the miniature Centrifugal Fast Analyzer. For different concentrations of drug, each rate of reaction decreased continuously with time, tending to converge on a single common value. The rate was most affected by the concentration of drug almost im...

Two enzyme immunoassays (ELISA) for mumps antibody detection using the Enzygnost (Germany) and Parotit-screen (Russia) were comparatively assayed using sera of randomly assigned 70 healthy young adult volunteers. The neutralization test (NT) was performed for all sera using mumps viruses (MVs) of the relevant strains Enders and Leningrad-3. The proportion of positive results was significantly h...

A rapid, specific, and sensitive modification of the dot immunobinding assay was compared with the standard enzyme immunoassay as a screening procedure for the detection of antibody in human or simian acquired immunodeficiency syndrome. Comparative testing with the available enzyme immunoassay procedures, either in commercial kit form or as provided by diagnostic laboratories, indicated excelle...

Methods The study included 407 non-pregnant women with high risk for toxoplasmosis, presented to the Infectious Diseases Clinic in Oradea in the period 01.01.2009-31.12.2012. By MEIA (Microparticle Enzyme Immunoassay) performed in Bioclinica laboratories, values of Toxoplasma IgM and IgG antibodies were determined and by EIA (Enzyme Immunoassay), the values of IgA Toxoplasma antibodies, as well...

The aim of this project was to produce guidance for a rationalised virological electron microscopy specimen testing policy for PHLS North West, to facilitate centralisation of a groupwide diagnostic electron microscopy service on a single site. Careful specimen selection to limit numbers and the groupwide use of commercially available enzyme immunoassays has allowed PHLS North West to reduce th...

A new homogeneous immunoassay (EMIT) for valproic acid was evaluated. Besides testing the manual version of this enzyme immunoassay, we also developed two mechanized procedures for centrifugal analyzers (the CentrifiChem and the COBAS system), which take less time and are more precise than the manual method. Within-assay precision (CV) was 4.5% with the manual technique and 2% with the analyzer...

Methods. Various laboratory tests were implemented in our lab, as screening or confirmatory tests, some in studies some as a routine diagnostic test, such are: Agglutinations tests: Rose Bengal test (RBT), Slide Agglutinations test, Standard tube test (SAT), Wright test, Antihuman globulin test (Coombs); 2-Mercaptoethanol test (2 ME); Complement fixation test (CFT); Indirect enzyme immunoassay ...

An enzyme immunoassay producing either a chromogenic or fluorogenic end product was developed and evaluated for detecting La Crosse viral antigen within mosquito pools. The enzyme immunoassay was found to be sensitive, detecting one infected mosquito within a pool of 100 mosquitoes, and specific, distinguishing between closely related California group viruses. Assays were completed within 5 h a...