The activities of three commercial products of papain were compared as supplied and after activation, and when used in the enzyme-serum method for rhesus (D) grouping. Certain dangers in activation became apparent, and accurate determination of the proportions of papain to anti-D appeared more important than indiscriminate activation without reference to the initial enzyme activity. A technique...

The objectives of this study were to (1) determine the effect exogenous fibrolytic enzyme derived from Trichoderma reesei on dry matter (DM) and neutral detergent fibre (NDF) degradability whole plant faba bean silage (Snowbird), (2) evaluate effects (FETR) DM NDF degradation kinetics silage, (3) compare difference between in vitro approach (DaisyII incubation method) situ assay-biological (nyl...

We describe an enzyme-immunoassay with photometric endpoint determination, suitable for the measurement of thyrotropin (TSH) in dried blood spotted on filter paper. Using reagents of a commercially available test kit provided for the measurement of thyrotropin in 200 microliter serum, we have adapted the method to the determination of thyrotropin in blood spots containing ca. 10 microliter bloo...