نتایج جستجو برای: enzymatic extraction
تعداد نتایج: 219125 فیلتر نتایج به سال:
This study offers a detailed mechanistic investigation of host-guest encapsulation behavior in a new enzyme-mimetic metal-ligand host and provides the first observation of a conformational selection mechanism (as opposed to induced fit) in a supramolecular system. The Ga4L4 host described features a C3-symmetric ligand motif with meta-substituted phenyl spacers, which enables the host to initia...
Mechanistic models for biochemical systems are frequently proposed from structural data. Site-directed mutagenesis can be used to test the importance of proposed functional sites, but these data do not necessarily indicate how these sites contribute to function. In this study, we applied an alternative approach to the catalytic mechanism of alkaline phosphatase (AP), a widely studied prototypic...
Unravelling the interaction of biological macromolecules with ligands and substrates at high spatial and temporal resolution remains a major challenge in structural biology. The development of serial crystallography methods at X-ray free-electron lasers and subsequently at synchrotron light sources allows new approaches to tackle this challenge. Here, a new polyimide tape drive designed for mix...
Reflections on advances and retreats in biochemistry have, over the years, generated several maxims. The late Efraim Racker’s ‘don’t waste clean thinking on dirty enzymes’ has been one of the most durable. On the occasion of a lecture in Jerusalem a decade ago, I framed a list of the ten ‘best’ maxims as ‘commandments’, which were later published as a ‘Guest Commentary’ in the Journal of Bacter...
Abstract Background and Objective: Increased antibiotic resistant strains and inadequacy of current vaccines against pneumococcal infections necessitate the study of novel protein antigens. It seems that minor autolysin of Streptococcus pneumoniae may have antigenicity. Thus, we aimed at cloning its gene for the first time. Material and Methods: After DNA extraction of Streptococcus pneumoniae ...
To realize the utilization of valorization buckwheat waste (BW), a two-stage thermal-chemical process was explored and evaluated to produce saccharides biochar. During first stage, BW underwent hydrothermal extraction (HTE) varying severity explore feasibility production; then, sum yields in liquid sample were compared. A higher 4.10% obtained at relatively lower factor (SF) 3.24 with byproduct...
Pectin from sugar beet (Beta vulgaris L.) (SBP) was extracted a waste (SBW) registering 4.4% (w/w) yield. SBP presented weight-average molar mass of 459 kDa, galacturonic acid content 52.2%, and low esterification degree (30%). The macromolecular characteristics revealed flexible extended coil chain conformation. main neutral sugars in were galactose (20.7%), mannose (5.0%), arabinose (3.60%) w...
The simultaneous extraction and enzymatic hydrolysis of sinapine from a mustard residue was designed for the recovery sinapic acid, high value-added phenolic acid. An initial screening allowed identification sinapoyl esterase activities in commercial cocktails (Depol 740 L, Ultraflo XL, Deltazym VR AC-100, Pectinase-PL “Amano”) mono-enzymatic solution rumen feruloyl esterase. These were not ver...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید